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黑曲霉(Aspergillus niger)β-D-葡萄糖苷酶的纯化及其性质
黑曲霉 β-D-葡萄糖苷酶
2007/12/21
摘要 利用垂直板凝胶制备电泳从黑曲霉(Aspergillus niger,AS 3.316)中分离提纯了β-D-葡萄糖苷酶(EC3.2.1.21),经凝胶电泳鉴定为单一带。酶作用的最适pH为4.4,在pH4.0—6.2稳定;最适温度65℃,热稳定性较好,于60℃保温4小时,活力保留80％。此酶作用于纤维二糖的Km值为6.09mM。聚丙烯酰胺薄层等电聚焦测得其pI值为5.5;用SDS凝胶电泳测得其分...
摘要应用启动子筛选载体(Promoter-trap vector) pLX2A构建了1个黑曲霉(Aspergillus niger)基因组文库。这个载体含有1个潮霉素B(Hy)磷酸转移酶编码基因(hph)，它能以启动子活性选择DNA片段。用这个基因组文库转化大肠杆菌，获得了80 000转化子菌落，其中94%含有插入片段。用这个基因库的质粒DNA转化黑曲霉，产生了53个抗潮霉素B(HyR)菌落。21...
Effects of Sucrose Concentration during Citric Acid Accumulation by Aspergillus niger
Aspergillus niger citric acid accumulation osmotic stress phosphotyrosine signal transduction
2010/4/21
A number of regulatory mechanisms are involved in increasing citric acid production under certain conditions. The accumulation of citric acid takes place under the high concentration of carbon sources...