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Impact of interspecific hybridization of T. pratense × T.medium and backcrossing on genetic variability of progeny
cytology FISH hybrids rDNA red clover zigzag clover
2016/12/8
Red clover (Trifolium pratense L.) is a high-quality fodder crop which has been hybridized successfully with its wild relative zigzag clover (T. medium L.). The aim of this study was to evaluate the g...
Comparison of Gene Expression between Cumulus Oocyte Complexes and Naked Oocytes by Suppression Subtractive Hybridization in Swine
Suppression Subtractive Hybridization (SSH) Gene Expression Cumulus Cells Porcine
2016/5/3
In the antral follicle phase, several layers of cumulus cells surround the oocyte and play an important support and regulation role in oocyte development and maturation via intercellular communication...
应用荧光原位杂交(FISH)技术研究黑叶猴染色体易位A Study of Chromosome Translocation of Francois Monkeyby Fluoresence in situ Hybridization (FISH)
黑叶猴 染色体易位 荧光原位杂交 人染色体特异探针
2008/1/30
摘要 本文应用染色体荧光原位杂交(FISH)技术,利用人9号和14号染色体特异探针,对深低温冻存和长期传代的黑叶猴细胞株染色体畸变进行了分析。确定在长期冻存和传代过程中,一些黑叶猴细胞在No.12和No.17染色体之间发生了易位,一条 No.17染色体发生断裂,断裂点在17q13,断裂片段17q13-17qter易位到一条 No.12染色体长臂末端,形成一条小的中着丝粒的和一条具较长长臂的衍生染色...
以胞质分裂阻滞法研究女性年龄与体细胞中21号染色体分离异常的关系Detection the Relation Between Chromosome 21 Missegregationand Age in Cytokinesis-blocked Female Lymphocytesby in situ Hybridization
细胞松弛素B 21号染色体 染色体不分离 年龄效应
2008/1/30
摘要21三体是人类最常见的先天性疾病，约95%患儿的超数21号染色体来源于母亲，且随母龄增加患儿出生率也随之增加。本文首次运用胞质分裂阻滞法(CB法)对不同年龄女性体细胞进行荧光原位杂交，对其21号染色体的分离情况进行分析。结果表明，随年龄的增加，女性体细胞中21号染色体不分离也随之增加，但21号染色体丢失无年龄效应, 且21号染色体不分离频率远高于其丢失。该结果表明，女性体细胞中21号染色体不分...
应用双色荧光原位杂交技术检测克氏综合征The Application of Dual-color Fluorescence in situ Hybridization to the Diagnosis of Klinefelter Syndrome
荧光原位杂交 克氏综合征 性染色体异常 DNA特异性探针
2007/12/30
摘要正常对照标准值4.17%）。用FISH 技术检测性染色体数目异常克氏综合征具有快速、敏感度高、信号强、背景低、多色等优点，故FISH 技术在产前诊断检测领域中显示其重要的应用价值和发展前景。
Abstract:The objective of the work is to study the technique of dual-color fluorescence in situ hybri...
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Intergeneric somatic hybridization between Brassica napus and Sinapis alba
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王幼平,陈建民
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J Integrative Plant Biology, 47(1): 84-91
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Identification f Wheat-Haynaldia villosa amphiploid ,addition ,substitution and translocation lines by in situ hybridization Using biotin labeled genomic DNA as a probe
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英文
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Chen ...
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Establishment and Identification of Cytoplasmic Male Sterility in Brassica napus by Intergeneric Somatic Hybridization.
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英文
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Hu Qiong,LI Yun-chang,MEI De-sheng,FANG Xiao-ping,Lise...
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Alien DNA introgression and wheat DNA rearrangements in a stable wheat line derived from the early generation of distant hybridization.
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Zhang Lian-quan,Liu Deng-cai,Yan Ze-ho...
Rapid Sex Identification of Chicken by Fluorescence In Situ Hybridization Using a W Chromosome-specific DNA Probe
Chicken W Chromosome XhoI family PCR FISH Sexing
2016/4/12
It has been known that the sex of chicken cells can be most accurately identified by fluorescence in situ hybridization (FISH). However, the presently available FISH has not been widely used for sex i...