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Vesicle Fusion Observed by Content Transfer Across a Tethered Lipid Bilayer
Endoplasmic reticulum-associated degradation (ERAD) ubiquitin-proteasome system (UPS) gp78/RNF45 Epidermal growth factor receptor (EGFR) Exosome Duffy antigen receptor for chemokine (DARC)
2016/5/25
Synaptic transmission is achieved by exocytosis of small, synaptic vesicles containing neurotransmitters across the plasma membrane. Here, we use a DNA-tethered freestanding bilayer as a target archit...
Covalent Attachment of Lipid Vesicles to a Fluid-Supported Bilayer Allows Observation of DNA-Mediated Vesicle Interactions
Caspian and Black Sea waters reverse-osmotic desalination Na-zeolite softening of the permeate
2016/5/25
Specific membrane interactions such as lipid vesicle docking and fusion can be mediated by synthetic DNA-lipid conjugates as a model for the protein-driven processes that are ubiquitous in biological ...
Diffusive Dynamics of Vesicles Tethered to a Fluid Supported Bilayer by Single-Particle Tracking
Calcium Macromolecular Substances 4-Chloro-7-nitrobenzofurazan DNA Lipid Bilayers Phosphatidylcholines Coated Materials, Biocompatible Nucleic Acid Hybridization Fluorescence Recovery After Photobleaching Diffusion
2016/5/24
We recently introduced a method to tether intact phospholipid vesicles onto a fluid supported lipid bilayer using DNA hybridization (Yoshina-Ishii, C.; Miller, G. P.; Kraft, M. L; Kool, E. T.; Boxer, ...
Early Steps of Supported Bilayer Formation Probed by Single Vesicle Fluorescence Assays
Adsorption Diffusion Lipid Bilayers Lipid Bilayers Liposomes Membrane Fluidity Membrane Fusion Membranes,Artificial Microscopy,Fluorescence,Multiphoton Motion Phosphatidylcholines Phosphatidylserines Xanthenes
2016/5/24
We have developed a single vesicle assay to study the mechanisms of supported bilayer formation. Fluorescently labeled, unilamellar vesicles (30-100 nm diameter) were first adsorbed to a quartz surfac...
Arrays of Independently-Addressable Supported Fluid Bilayer Membranes
Arrays Independently Addressable Fluid Bilayer Membranes
2016/5/23
A surface detector array device suitable for use with a biosensor is disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions...
Cell adhesion to protein-micropatterned-supported lipid bilayer membranes
micropatterning microcontact printing of proteins supported lipid bilayers cell adhesion endothelial cells
2016/5/23
A new method for constructing controlled interfaces between cells and synthetic supported lipid bilayer membranes is reported. Microcontact printing is used to define squares and grid lines of fibrone...
Formation of Supported Lipid Bilayer Composition Arrays by Controlled Mixing and Surface Capture
tetrafluorophosphite mean amplitudes of vibration bond properties
2016/5/23
Virtually all structures in living cells are defined by lipid bilayers. Many molecular components can diffuse along these 2-dimensional structures, sampling all or part of the membrane, and this diffu...
Patterning Barriers to Lateral Diffusion in Supported Lipid Bilayer Membranes by Blotting and Stamping
Patterning Barriers Lateral Diffusion Lipid Bilayer Membranes Blotting Stamping
2016/5/23
Two new methods are introduced for patterning fluid lipid bilayer membranes on solid supports. These methods are based on the observation that supported membranes undergo self-limiting lateral expansi...
Substrate-Membrane Interactions:Mechanisms for Imposing Patterns on a Fluid Lipid Bilayer Membrane
X-ray resonant exchange scattering X-ray absorption fine structure surface contamination PuSb
2016/5/23
A variety of new techniques are emerging that require the use of a patterned substrate to impose micropartitions on a supported fluid bilayer membrane. The barrier-forming characteristics of aluminum ...
Electric Field-Induced Critical Demixing in Lipid Bilayer Membranes
Cardiolipins Electric Stimulation Electricity Fluorescent Dyes Lipid Bilayers Membranes, Artificial Microscopy, Fluorescence Models, Chemical Models, Molecular hosphatidylcholines Phosphatidylethanolamines Thermodynamics
2016/5/23
Electric fields can induce lateral reorganization of lipids in fluid bilayer membranes. The resulting concentration profiles readily are observed in planar-supported bilayers by epifluorescence micros...
Electric Field-Induced Reorganization of Two-Component Supported Bilayer Membranes
Electric Field-Induced Two-Component Bilayer Membranes
2016/5/23
Application of electric fields tangent to the plane of a confined patch of fluid bilayer membrane can create lateral concentration gradients of the lipids. A thermodynamic model of this steady-state b...