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Effect of Antioxidant Flavonoids (Quercetin and Taxifolin) on In vitro Maturation of Porcine Oocytes
Porcine Oocyte Antioxidants Quercetin Reactive Oxygen Species [ROS]
2016/5/19
Quercetin (QT) and taxifolin (TF) are structurally similar plant-derived flavonoids that have antioxidant properties and act as free radical scavengers. The objective of this study was to investigate ...
Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro
MAPK DNA fragmentation apoptosis Bax
2015/6/24
Oocyte ageing is a complex of processes that occur when matured in vitro oocytes are, after reaching the metaphase II stage, exposed to further in vitro culture. Aged oocytes remaining at the metaphas...
Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
Pig Skeletal Muscle Satellite Cell Isolation Culture Characterization
2016/5/18
The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, whi...
Knock-in of Enhanced Green Fluorescent Protein or/and Human Fibroblast Growth Factor 2 Gene into β-Casein Gene Locus in the Porcine Fibroblasts to Produce Therapeutic Protein
Knock-in Homologous Recombination Therapeutic Proteins Porcine β-casein Gene
2016/5/17
Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjectio...
Bacillus subtilis Protects Porcine Intestinal Barrier from Deoxynivalenol via Improved Zonula Occludens-1 Expression
Zonula Occludens-1 Porcine Intestinal Epithelial Cell Line (IPEC-J2)
2016/5/16
Intestinal epithelial cells (IECs) forming the barrier for the first-line of protection are interconnected by tight junction (TJ) proteins. TJ alteration results in impaired barrier function, which ca...
Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation
Peripheral Blood Mononuclear Cells (PBMC) Polyinosinic:Polycytidylic Acid
2016/5/16
Investigating gene expression of immune cells of whole blood or peripheral blood mononuclear cells (PBMC) under polyinosinic:polycytidylic acid (poly I:C) stimulation is valuable for understanding the...
Phosphorylation Status of RNA Polymerase II Carboxyl-terminal Domain in Porcine Oocytes and Early Embryos
RNA Polymerase II Porcine Oocyte Embryos α-Amanitin
2016/5/10
Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryoni...
Effects of Oviductal Fluid, Culture Media and Zona Pellucida Removal on the Development of Porcine Embryos by Nuclear Transfer
Oviductal Fluid Nuclear Transfer Culture Media Zona Pellucida Porcine
2016/4/29
The aim of this study was to compare the effects of oviductal fluid, porcine zygote medium (PZM)-3, PZM-4 and PZM-5, and modified PZM-5 culture media, and determine the effects of zona pellucida (ZP) ...
Relationship between Developmental Ability and Cell Number of Day 2 Porcine Embryos Produced by Parthenogenesis or Somatic Cell Nuclear Transfer
Blastomeric Fragmentation Developmental Ability Embryo Quality Parthenogenesis Porcine Somatic Cell Nuclear Transfer
2016/4/29
In vitro produced porcine embryos have potential application in reproductive biotechnology. However, their development potential has been very low. This study evaluated the in vitro developmental abil...
The Effect of the Addition and Removal of Various Cryoprotectants on the Nuclear Maturation and ATP Content of Immature Porcine Oocytes
Cryoprotectant Porcine Oocytes ATP Content
2016/4/29
This study was undertaken to investigate the influence of exposure and removal of four different cryoprotectants (CPAs) on the ATP content of cumulus cell-enclosed (COs) and cumulus cell-denuded (DOs)...
Efficient Derivation and Long Term Maintenance of Pluripotent Porcine Embryonic Stem-like Cells
Porcine Embryonic Stem Cells Pluripotent IVF Basic Fibroblast Growth Factor
2016/4/28
Porcine embryonic stem (ES) cells have a great potential as tools for transgenic animal production and studies of regulation of differentiation genes. Although several studies showed successful deriva...
Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection
Lipofection Porcine Developmental Competence Somatic Cell Nuclear Transfer (SCNT)
2016/4/27
The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro deve...
Effects of Sperm Membrane Disruption and Electrical Activation of Oocytes on In vitro Development and Transgenesis of Porcine Embryos Produced by Intracytoplasmic Sperm Injection
Intracytoplasmic Sperm Injection GFP Transgenic Pigs
2016/4/27
The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species ...
Parthenogenetic Activation of Porcine Oocytes and Isolation of Embryonic Stem Cells-like Derived from Parthenogenetic Blastocysts
Parthenogenetic Activation Oocyte Embryonic Stem Cells Porcine
2016/4/26
These experiments were carried out to optimize the parameters of electrical activation, methods of parthenogenetic activation and embryo culture in vitro and meanwhile to isolate embryonic stem cells-...
2, 4-Thiazolidindion Induced Plasticity of Myoblast (C2C12) and Satellite Cells (Porcine) - A Comparative Study
Adipogenesiss Adipogenesis Myoblast (C2C12) Satellite Cells (Porcine) Pax-7 2, 4-Thiazolidindion
2016/4/26
This study was conducted to determine the difference between satellite cells (porcine) and myoblasts (C2C12) in their differentiation under the influence of 2, 4-thiazolidindion. C2C12 myoblast cells ...