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牙鲆(Paralichthys olivaceus)丝氨酸蛋白酶Ⅰ-1基因多态性
丝氨酸蛋白酶基因 牙鲆 鳗弧菌 多态性
2015/12/2
采用重测序方法，利用牙鲆(Paralichthys olivaceus)野生个体，扩增了牙鲆丝氨酸蛋白酶(Serine protease) I-1基因的编码区(CDS)和2605 bp的启动子序列。在牙鲆丝氨酸蛋白酶I-1基因(PoSP I-1)编码区中筛选出9个单核苷酸多态性位点(SNPs)，同时在其启动子区筛选出28个SNP位点。对感染鳗弧菌的抗病牙鲆和易感牙鲆进行了部分SNP位点的分型，通过...
通过统计学、酶联免疫学等方法研究3个池塘中养殖牙鲆(Paralichthys olivaceus)的生长状况、血清四碘甲状腺素(T4)和三碘甲状腺原氨酸(T3)、生长激素(GH)、类胰岛素生长因子-I(IGF-Ⅰ)和温度的周年变化规律。结果显示，在365 d的养殖过程中，3个池塘牙鲆体重和体长持续增长且体重日增长率和体长日增长率在温度较高的夏末秋初出现较高值。3个池塘牙鲆血清中T3、T4含量变化整...
Effects of Dietary Supplementation of Barodon, an Anionic Alkali Mineral Complex, on Growth Performance, Feed Utilization, Innate Immunity, Goblet Cell and Digestibility in Olive Flounder (Paralichthys olivaceus)
Growth Gastrointestinal Histology Nutrient Digestibility Innate Immunity
2016/5/16
A 15-wk feeding trial was conducted to examine the supplemental effects of Barodon on growth performance, gastrointestinal histology, feed digestibility and innate immunity in olive founder. A basal c...
以牙鲆为研究对象，利用染色体步移（Genome walking）获得了Activin两个β亚基基因的上游部分启动子序列，并对其进行了转录调控元件的生物信息学预测分析。获得了Activin βA和βB两个亚基的启动子部分序列，长度分别约为2.7 kb和2.4 kb；在预测的Activin βB转录起始位点（+1位）的上游31 bp处有1个典型的TATA box，而在Activin βA中未发现TAT...
采用微卫星遗传标记技术对三倍体牙鲆群体及二倍体对照群体进行比较分析。取2012年冷休克诱导和培育至11月龄的三倍体及同期二倍体对照牙鲆个体各32尾，通过高盐法提取肌肉组织总DNA；利用筛选得到的21对微卫星引物进行PCR扩增，并经14%聚丙烯酰胺凝胶电泳分型后，进行人工判读和分析。结果显示，21个微卫星位点均为多态，二倍体和三倍体群体的平均等位基因数（A）、基因型总数、平均有效等位基因数（ae）、...
Effects of Dietary Scutellaria baicalensis Extract on Growth, Feed Utilization and Challenge Test of Olive Flounder (Paralichthys olivaceus)
Olive Flounder (Paralichthys olivaceus) Scutellaria baicalensis Extract Dietary Additive
2016/5/11
Effects of dietary Scutellaria baicalensis extract (SBE) on growth, feed utilization and challenge test of olive flounder (Paralichthys olivaceus) were determined. Six hundred thirty fish averaging 5....
Effects of Dietary Lipid Source and Level on Growth Performance, Blood Parameters and Flesh Quality of Sub-adult Olive Flounder (Paralichthys olivaceus)
Dietary Lipid Olive Flounder Paralichthys olivaceus Fatty Acid Flesh Quality
2016/5/10
This study was conducted to investigate the effects of dietary lipid source and level on growth performance, blood parameters, fatty acid composition and flesh quality of sub-adult olive flounder Para...
Compensatory Growth of Grower Olive Flounder (Paralichthys olivaceus) with Different Feeding Regime at Suboptimal Temperature
Olive Flounder (Paralichthys olivaceus) Compensatory Growth Grower Feed Deprivation
2016/5/9
Compensatory growth of grower olive flounder (Paralichthys olivaceus) was determined at suboptimal temperature (13.0±1.9°C). Fifteen fish averaging 201.1 g per tank were distributed into 18 of 300 L f...
Effect of Dietary Composition with Different Feeding Regime on Compensatory Growth of Juvenile Olive Flounder Paralichthys olivaceus
Olive Flounder (Paralichthys olivaceus) Compensatory Growth Different Diets Feeding Regimes
2016/5/6
Compensatory growth of juvenile olive flounder Paralichthys olivaceus fed different diets with different feeding regime was compared. Four hundred fifty fish (twenty five fish per tank) were randomly ...
Single Nucleotide Polymorphisms of the GnRHR Gene Associated with Reproductive Traits of Japanese Flounder (Paralichthys olivaceus)
Japanese Flounder GnRHR SNPs Diplotype Reproductive Traits
2016/5/6
Gonadotropin-releasing hormone receptor (GnRHR) gene is expressed at the anterior pituitary gland and plays a key role in gonad development. This study aimed to investigate molecular genetic character...
Effect of Dietary Protein and Lipid Levels on Compensatory Growth of Juvenile Olive Flounder (Paralichthys olivaceus) Reared in Suboptimal Temperature
Olive Flounder (Paralichthys olivaceus) Compensatory Growth Dietary Protein Dietary Lipid Suboptimal Temperature
2016/5/6
Effect of dietary protein and lipid levels on compensatory growth of juvenile olive flounder (Paralichthys olivaceus) was determined in suboptimal temperature (13.41.42C). Five hundred...
Effect of Intermittent Feeding on Growth, Feed Utilization and Body Composition of Subadult Olive Flounder Paralichthys olivaceus in Suboptimal Temperature
Olive Flounder (Paralichthys olivaceus) Intermittent Feeding Subadult Suboptimal Temperature
2016/5/6
The effect of intermittent feeding on growth, feed utilization and body composition of subadult olive flounder fed extruded pellet (EP) in suboptimal temperature (13.52.10C) was determ...
鱼类胚胎冷冻保存经过多年研究，尽管有所突破，但目前仍未达到应用水平，其主要原因是由于鱼类胚胎体积大、卵膜通透性差等特点，导致传统的平衡法，无法使抗冻剂足够、有效的进入胚胎内部起到保护胚胎的作用。而在鱼类胚胎冷冻保存中利用显微注射技术，可直接将抗冻剂注射到鱼类胚胎卵黄囊内，这一定程度上弥补了普通平衡法的不足，提高抗冻剂对胚胎的保护程度。本研究主要对抗冻剂的选择、抗冻剂对牙鲆胚胎的注射剂量、注射浓度、...
Improved Apparent Digestibility Coefficient of Protein and Phosphorus by Supplementation of Microbial Phytase in Diets Containing Cottonseed and Soybean Meal for Juvenile Olive Flounder (Paralichthys olivaceus)
Microbial Phytase Olive Flounder Cottonseed Meal Soybean Meal
2016/4/28
This study was conducted to investigate the effects of phytase in diets containing cottonseed and soybean meal (CS) on growth performance, feed utilization and digestibility of protein and phosphorus ...
Long Term Feeding Effects of Dietary Dehulled Soybean Meal as a Fish Meal Replacer in Growing Olive Flounder Paralichthys olivaceus
Dehulled Soybean Meal (DHSM) Fish Meal (FM) Replacer Long Term Olive Flounder
2016/4/27
This experiment was conducted to evaluate the long term effects of dehulled soybean meal (DHSM) as fish meal (FM) replacer in the diet for olive flounder, Paralichthys olivaceus, for 42 wk at a commer...