搜索结果: 1-3 共查到“农学 RT-PCR-RFLP”相关记录3条 . 查询时间(0.062 秒)
本研究旨在建立猪瘟病毒野毒株和兔化弱毒疫苗株RTPCRRFLP鉴别检测方法。根据Shimen株设计1对特异性引物，建立猪瘟病毒 RTPCRRFLP检测方法；对20份疑似猪瘟临床样品进行检测，并对检出的山东8株流行野毒株和2株疫苗株PCR产物进行克隆与序列分析，验证上述方法。结果RTPCR扩增片段为825 bp，产物经RFLP分析，野毒株的PCR产物能被ApaⅠ酶切为322 bp和503 ...
本研究旨在建立猪瘟病毒野毒株和兔化弱毒疫苗株RT-PCR-RFLP鉴别检测方法。根据Shimen株设计1对特异性引物，建立猪瘟病毒 RT-PCR-RFLP检测方法；对20份疑似猪瘟临床样品进行检测，并对检出的山东8株流行野毒株和2株疫苗株PCR产物进行克隆与序列分析，验证上述方法。结果RT-PCR扩增片段为825 bp，产物经RFLP分析，野毒株的PCR产物能被ApaⅠ酶切为322 bp和503 ...
Detection and Characterization of Plum Pox Potyvirus（PPV）by DAS-ELISA and RT-PCR / RFLP Analysis in Turkey
Enzyme-linked immunosorbent assay plum pox potyvirus restriction analysis reverse transcription-polymerase chain reaction
2009/1/13
This study was conducted to determine the presence of plum pox virus (PPV) (family Potyviridae, genus Potyvirus) in different regions of Turkey and to characterize PPV isolates by serological and mol...