搜索结果: 1-8 共查到“作物学 Protoplasts”相关记录8条 . 查询时间(0.056 秒)
Studies on Culture of Cells and Tissues of Crop Plants:III. Isolation of rice mesophyll protoplasts with a new cell wall lytic enzyme "funcelase"
Cell wall Cellulase "Onozuka" R-10 Funcelase Mesophyll Oryza sativa L
2009/8/6
Rice mesophyll protoplasts were isolated with ease and with high yield from expanded 2nd leaves, which were grown on agar plates under 23°C, 16.5 W/m2 for 25 days, by using a new cell wall lytic enzym...
Studies on Culture of Cells and Tissues of Crop Plants:I. Survey on enzymatic isolation and culture of rice leaf sheath protoplasts
BSA Cell activity Cellulase "Onozuka" R-10 Protoplasts MS-medium Rice leaf sheath
2009/8/6
To establish a method for isolation and culture of rice protoplasts, enzyme solution compositions, culture conditions, and the selection of cultivars and tissues as materials were examined. The result...
Morphological Study of Soybean Protoplasts in Early Electrofusion Process
Electrofusion Fusion process Soybean protoplast Ultrastructure
2009/8/6
The present study is intended to analyze the early response of protoplasts isolated from soybean suspension cells to electrofusion. It was observed by light microscopy that protoplasts formed a pearl ...
Studies on Cell Wall Regeneration and Cell Division in Soybean Protoplasts Using Fluorescence and Scanning Electron Microscopy
Calcofluor White Cell wall formation Cell division SEM Soybean protoplast
2009/8/5
Protoplasts were enzymatically isolated from soybean suspension cells and were cultured in a liquid medium. The reappearance of cell wall at the surface of naked protoplasts was studied by fluorescenc...
Ultrastructural Analysis of Cell Wall Formation and Cell Division of Soybean Protoplasts
Cell division Cell wall formation Protoplasts Soybean TEM Ultrastructure
2009/8/4
Protoplasts of soybean (Glycine max (L.) Merr.) were isolated from suspension cultures and morphological changes during culture were observed using transmission electron microscopy. The regeneration ...
An Efficient Method for Callus Formation of Protoplasts from Peanut (Arachis hypogaea L.) Leaf Mesophyll
Callus formation Mesophyll protoplast Peanut Protoplast culture
2009/7/30
High yields of viable protoplasts were obtained from leaf mesophyll of 10-day-old peanut seedlings (Arachis hypogaea L. var. Lokal). An enzyme solution containing 1% Cellulase YC, 2% Meicelase P-1, 0....
Evaluation of Preparation Methods for Scanning Electron Microscopic Observation of Plant Protoplasts
Dehydration Fixation method Petunia Protoplast Protoplast size Rice
2009/7/28
Preparation methods for scanning electron microscopy (SEM) were studied with petunia and rice protoplasts. Fixation schedules to sustain protoplast size during alcohol dehydration were examined. When ...
Ultrastructural Changes of Chloroplasts in Peanut Mesophyll Protoplasts Treated with Electric Fields
Chloroplast division Chloroplast fusion Electric fields Mesophyll protoplasts Peanut
2009/7/28
Ultrastructural changes in chloroplasts of peanut (Arachis hypogaea L. var. Lokal) seedling mesophyll cells and mesophyll protoplasts treated with electric fields were investigated. Generally, chloro...