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Cloning and Expression of Yak Active Chymosin in Pichia pastoris
Active Chymosin Yak Expression Pichia pastoris
2016/11/10
Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain c...
Molecular Cloning and mRNA Expression of the Porcine Insulin-responsive Glucose Transporter (GLUT4)
GLUT4 IR Skeletal muscle Porcine
2016/5/4
Insulin-responsive glucose transporter 4 (GLUT4) is a member of the glucose transporter family and mainly presents in skeletal muscle and adipose tissue. To clarify the molecular structure of porcine ...
Cloning and characterization of the first actin gene in Chinese oak silkworm, Antheraea pernyi
Antheraea pernyi actin cloning expression pattern
2011/1/11
The first actin gene in Chinese oak silkworm, Antheraea pernyi (Apactin) was cloned and characterized. Remarkably, two types of the Apactin cDNA clones were isolated from the pupal cDNA library, which...
Cloning of Perivitelline Membrane Protein; ZP1 in Turkey (Meleagris gallopavo)
egg envelope fibrogenesis perivitelline membrane turkey ZP1
2009/7/29
In aves, the perivitelline membrane is an extracellular matrix surrounding the oocyte at the time of ovulation, and is composed of several glycoproteins belonging to the zona pellucida (ZP) gene famil...
Cloning and Expression of Cellular Glutathione Peroxidase (GPX1) in Japanese Quail (Coturnix japonica)
antioxidant enzyme cellular glutathione peroxidase Japanese quail mRNA expression selenocysteine
2009/7/29
Glutathione peroxidase 1 (cellular glutathione peroxidase, EC 1.11.1.9 (GPX1)) is an antioxidant enzyme that plays an important role in scavenging reactive oxygen species to protect cells from oxidati...
Cloning and Comparison of Prolactin Promoter in Galliformes
Galliformes prolactin promoter
2009/7/29
To investigate the mechanism regulating transcription of the prolactin (PRL) gene in avian species, the PRL promoter region in Ceylon junglefowl, Japanese quail, ring-necked pheasant, turkey, Indian p...
家蚕核型多角体病毒山东株p35基因的克隆及序列分析Cloning and Sequencing of p35 Gene from Bombyx mori Nuclear Polyhedrosis Virus Strain Shandong
家蚕核型多角体病毒山东株 p35基因 克隆 序列分析
2008/1/16
摘要根据家蚕核型多角体病毒T3株p35基因非编码区序列设计一对特异性引物,应用PCR技术从家蚕核型多角体病毒山东株中扩增得到一条1 080 bp的片段。测序结果表明,该片段含有p35基因,开放阅读框长900 bp,编码299个氨基酸,分子量为34.91kDa,pI=6.4。序列分析表明,BmNPV山东株的p35基因与T3株存在差异。
Abstract:According to the 5′ an...
Cloning and Expression of the Duck Leptin Gene and the Effect of Leptin on Food Intake and Fatty Deposition in Mice
Duck Leptin Expression Food Intake Fatty Deposition
2016/4/25
Leptin is the adipocyte-specific product of the obese gene and plays a major role in food intake and energy metabolism. Leptin research was mainly focused on mammalian species, but understanding of le...
The Porcine FoxO1, FoxO3a and FoxO4 Genes: Cloning, Mapping, Expression and Association Analysis with Meat Production Traits
Pig FoxO1 FoxO3a FoxO4 Mapping Association Analysis
2016/4/25
FoxO1, FoxO3a and FoxO4 belong to the FoxO gene family, which play important roles in the PI3K/PKB pathway. In this study, we cloned the porcine FoxO1, FoxO3a and FoxO4 sequences and assigned them to ...
Microsurgical nuclear transfer by intraooplasmic karyoplast injection as an alternative embryo reconstruction method in somatic cloning of pigs and other mammal species; application value of the method and its technical advantages: a review
somatic cloning microsurgical nuclear transfer karyoplast intraooplasmic injection chromatin remodelling epigenetic reprogramming
2015/6/1
The introduction of cell nuclei into enucleated recipient cells, beyond enucleation, is the most significant stage of somatic cloning procedure. Microsurgical transfer of somatic nuclei can be an alte...
Cloning and Characterization of Liver cDNAs That Are Differentially Expressed between Chicken Hybrids and Their Parents
mRNA Differential Display Differential Gene Expression Heterosis Chicken
2016/4/20
Using mRNA differential display technique, we investigated differential gene expression in hybrids relative to their parents in a diallel cross involving four chicken breeds in order to provide an ins...