搜索结果: 1-15 共查到“3T3-L1”相关记录45条 . 查询时间(0.069 秒)
高温对3T3-L1前体脂肪细胞凋亡和脂肪沉积的影响
高温 3T3-L1前体脂肪细胞 凋亡 脂肪合成 脂肪分解
2021/6/18
本试验旨在研究高温对3T3-L1前体脂肪细胞凋亡和脂肪沉积的影响。选择3T3-L1前体脂肪细胞为研究材料,分别以37.0和41.5℃处理细胞,4 d后观察细胞凋亡情况,分化处理6 d后,油红O染色观察脂滴的数量,采用实时荧光定量PCR(qRT-PCR)技术测定热休克蛋白家族基因热休克蛋白60(HSP60)、热休克蛋白70(HSP70)、热休克蛋白90(HSP90),细胞凋亡相关基因半胱氨酸天冬氨酸...
GLP-1对3T3-L1前脂肪细胞增殖及脂联素的影响
肥胖 3T3-L1前脂肪细胞 胰高血糖素样肽-1 细胞增殖 脂联素 实验研究
2020/7/24
观察胰高血糖素样肽-1(GLP-1)对小鼠3T3-L1前脂肪细胞增殖及脂联素的影响。方法不同浓度GLP-1作用于诱导分化完全的3T3-L1前脂肪细胞,用细胞计数试剂盒(CCK-8)比色法测定GLP-1对细胞增殖的影响,酶联免疫吸附试验(ELISA)法测定细胞上清液中脂联素含量。
果糖通过激活Akt信号通路促进3T3-L1细胞的脂肪分化
果糖 3T3-L1前脂肪细胞 细胞分化 Akt信号通路
2020/9/17
研究果糖(fructose)对3T3-L1前脂肪细胞分化过程的影响及其作用机制。方法:对体外培养的3T3-L1前脂肪细胞给予鸡尾酒法诱导脂肪分化,并使用1 g/L的果糖进行诱导干预。油红O染色法定量分析细胞内的脂质含量;RT-qPCR法检测脂肪分化过程中脂滴包被蛋白2(Plin2)、CCAAT/增强子结合蛋白(C/EBP)α和C/EBPβ的mRNA表达水平;Western blot检测脂肪分化标志...
二甲双胍作用于3T3-L1前脂肪细胞诱导阶段对v-SNAREs蛋白家族mRNA表达的影响
糖尿病 二甲双胍 囊泡相关可溶性N-乙酰基亚胺敏感性因子附着蛋白受体 囊泡相关膜蛋白 3T3-L1前脂肪细胞
2020/7/23
观察3T3-L1前脂肪细胞诱导分化过程中二甲双胍对囊泡相关膜蛋白(VAMPs)mRNA表达变化,探讨二甲双胍促进葡萄糖转运子4(GLUT4)转运中的作用机制。方法在3T3-L1前脂肪细胞诱导分化过程中,分别持续给予不同浓度的二甲双胍(0、0.5 mmol/L、1 mmol/L、2 mmol/L)干预12 d后,比较不同浓度的二甲双胍对AMPK、GLUT4及VAMP2、VAMP3、VAMP4、VAM...
探讨Apelin-13对3T3-L1前体脂肪细胞增殖与分化的影响及其可能的作用机制。方法 体外培养3T3-L1前体脂肪细胞。取对数生长期细胞,分别予以不同浓度Apelin-13进行干预,四甲基偶氮唑盐(MTT)法检测Apelin-13对3T3-L1前体脂肪细胞增殖的影响。“经典鸡尾酒法”诱导3T3-L1前体脂肪细胞分化。将对数生长期细胞分为实验组和对照组。实验组分别于诱导分化第2、4、6、8天更换...
Lignosulfonic acid promotes hypertrophy in 3T3-L1 cells without increasing lipid content and increases their 2-deoxyglucose uptake
Adipogenesis Diabetes Dietary Fiber Glucose Uptake Lignosulfonic Acid
2017/3/7
Adipose tissue plays a key role in the development of obesity and diabetes. We previously reported that lignosulfonic acid suppresses the rise in blood glucose levels through the inhibition of α-gluco...
Heat Shock Protein Augmentation of Angelica gigas Nakai Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes
Angelica gigas Nakai Heat Shock Heat Shock Protein 3T3-L1 Adipogenesis
2016/5/19
There is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation throug...
Ramie Leaf Extracts Suppresses Adipogenic Differentiation in 3T3-L1 Cells and Pig Preadipocytes
Ramie Leaf Preadipocytes Proliferation Differentiation Obesity
2016/11/10
The present study was carried out to evaluate the anti-obesity effect of different concentrations of extracts of hot air-dried ramie leaf (HR) and freeze-dried ramie leaf (FR) in 3T3-L1 cells and pig ...
HISTONE DEACETYLASE INHIBITORS TRICHOSTATIN A (TSA) AND SULFORAPHANE (SFN) MODULATE VITAMIN D RESPONSIVE CYP24 GENE EXPRESSION in 3T3-L1 PREADIPOCYTES
TRICHOSTATIN A (TSA) SULFORAPHANE (SFN), CYP24 3T3-L1 PREADIPOCYTES
2014/11/7
Vitamin D plays an important role in preserving healthy bones, and has additional roles in the body, including modulation of cell growth, differentiation, neuromuscular and immune function, and anti-i...
Vitamin D Metabolites Inhibit Adipocyte Differentiation in 3T3-L1 Preadipocytes
3T3-L1 preadipocytes inhibition of differentiation
2014/11/7
The rising prevalence of obesity in the past 20 years underscores the need to
understand the biology and key regulatory processes involved in the
development of obesity. Adipogenesis, or the form...
表达谱芯片分析KCTD15对3T3-L1前体脂肪细胞的影响
基因 3T3-L1前体脂肪细胞 基因芯片 肥胖
2014/5/21
通过筛选分析3T3-L1前体脂肪细胞中KCTD15基因敲低后的差异表达基因研究其细胞生物学功能,探讨KCTD15基因与肥胖发生的关联。 方法 ①运用RNA干扰技术在3T3-L1前体脂肪细胞中特异性敲低KCTD15基因表达,并用半定量RT-PCR技术检测KCTD15基因敲低效率。②用Illumina表达谱芯片检测基因表达。③筛选差异表达基因后,运用Gene Ontology(...
转染促甲状腺素受体(TSHR)shRNA的3T3-L1脂肪细胞以牛促甲状腺素(TSH)刺激,酶联免疫吸附法(ELISA)检测培养基中肿瘤坏死因子α(TNF-α)水平,Western印迹法检测胰岛素受体底物1(IRS-1)的蛋白表达,免疫沉淀法检测IRS-1酪氨酸磷酸化。结果显示,1 mIU/ml TSH明显增加3T3-L1脂肪细胞TNF-α分泌[(341.85±12.00对522.67±36.22...
探讨JAZF1(juxtaposed with another zinc finger gene 1)基因对3T3-L1脂肪细胞蛋白激酶B(Akt)和AMP活化的蛋白激酶(AMPK)信号通路的影响。方法 构建pGenesil-JAZF1shRNA重组质粒,转染3T3-L1脂肪细胞后分别用胰岛素或利拉鲁肽处理细胞。2-脱氧-3H-D-葡萄糖掺入法检测细胞的葡萄糖摄取,Western印迹法检测各组细胞...
胰升糖素样肽1通过PKA途径促进3T3-L1脂肪细胞内脂素的表达
胰升糖素样肽1 内脂素 脂肪细胞 糖尿病
2014/4/9
检测胰升糖素样肽1(GLP-1)是否调节内脂素的合成,并探讨其作用机制。方法以3T3-L1分化成熟脂肪细胞为模型,GLP-1干预后,提取总RNA定量检测内脂素转录水平,收集培养液酶联免疫法检测内脂素分泌水平;PKA通路抑制剂H89预处理30 min,检测内脂素的转录水平。结果GLP-1呈剂量依赖性增加3T3-L1细胞表达内脂素,10-10mol/L时显著提高(P<0.05), 10-9mo...