搜索结果: 16-30 共查到“知识库 核酸生物化学”相关记录324条 . 查询时间(5.269 秒)
Characterization of the Overall and Internal Dynamics of Short Oligonucleotides by Depolarized Dynamic Light Scattering and NMR Relaxation Measurements
Overall and Internal Dynamics Short Oligonucleotides Depolarized Dynamic Light Scattering NMR Relaxation
2016/5/23
The dynamics of three synthetic oligonucleotides d(CG)4, d(CG)6, and d(CGCGTTGTTCGCG) of different length and shape were studied in solution by depolarized dynamic light scattering (DDLS) and time-res...
Electrostatic Interactions in Wild Type and Mutant Recombinant Human Myoglobins
SUBSTANCE-P GABA PROLACTIN ANTERIOR PITUITARY HYPOTHALAMIC FRAGMENTS ANTI-SUBSTANCE-P SERUM
2016/5/20
Residue Val68 in human myoglobin has been replaced by Asn, Asp, and Glu with site-directed mutagenesis. Purified proteins were characterized by isoelectric focusing and by absorption, CD, and NMR spec...
Ligand and Proton Exchange Dynamics in Recombinant Human Myoglobin Mutants
Mb myoglobin n.m.r. nuclear magnetic resonance fwhm full width at half maximum TSP sodium 3-trimethyl silyl propionate DSS 4,4-dimethyl-4-sila pentane-1-sulfonate
2016/5/20
Site-specific mutants of human myoglobin have been prepared in which lysine 45 is replaced by arginine (K45R) and aspartate 60 by glutamate (D60E), in order to examine the influence of these residues ...
Synthesis of a Thymidine Phosphoramidite Labelled with 13C at C6: Relaxation Studies of the Loop Region in a 13C Labelled DNA Hairpin
Thymidine Phosphoramidite Labelled 13C C6 Relaxation Studies 13C Labelled DNA Hairpin
2016/5/20
A thymidine phosphoramidite labelled at C6 with 13C has been synthesized, and incorporated into a synthetic oligonucleotide, d(CGCGT*T*GT*T*CGCG), which adopts a hairpin conformation. NMR relaxation m...
Chemically Induced Dynamic Nuclear Polarization Studies of Yeast tRNAPhe
Chemically Induced Dynamic Nuclear Polarization Studies Yeast tRNAPhe
2016/5/20
Chemically induced dynamic nuclear polarization (CIDNP) has been observed from yeast tRNAPhe following reaction with photoexcited riboflavin. At 20 OC, several resonances of tRNA in the native form sh...
UV Excimer Laser Chemically Induced Dynamic Nuclear Polarization of Amino Acids at 360 MHz
UV Excimer Laser Chemically Dynamic Nuclear Polarization Amino Acids 360 MHz
2016/5/20
A system has been developed which permits ultraviolet irradiation of samples in the probe of a 360 MHz nmr spectrometer using an excimer laser (A= 249 or 308 nm). This system is ideally suited for stu...
Laser Chemically Induced Dynamic Nuclear Polarization Study of the Reaction between Photoexcited Flavins and Tryptophan Derivatives at 360 MHz
Laser Chemically Induced Dynamic Nuclear Polarization Study Photoexcited Flavins Tryptophan Derivatives 360 MHz
2016/5/20
Chemically induced dynamic nuclear polarization (CIDNP) is generated when tryptophan (Trp), its derivatives,or Trp-containing peptides react with photoexcited flavins in a 360-MHz NMR spectrometer. In...
Characterization of yeast U14 snoRNA interactions required for rRNA processing, and development of a novel in vivo rDNA system for dissecting ribosome biogenesis
U14 small nucleoli ribosomal RNA DNA bases yeast elements
2014/12/11
U14 small nucleolar RNA (snoRNA) is required for processing of 18S ribosomal RNA. It was hypothesized that U14 might base pair with 18S RNA through two highly conserved U14 sequence elements known as ...
用Trizol法、SDS-LiCl法、改良CTAB法分别对对叶榕（Ficus hispida）、聚果榕（Fracemosa）、高榕（F altissima）、苹果榕（Foligadon）４种榕树叶片提取总RNA．通过紫外吸光值及甲醛变性凝胶电泳检测，结果显示：Trizol法只适合于对叶榕总RNA的提取，在其余３种榕树中不能提得；SDS-LiCl法可以从除苹果榕外的其余３种榕树中提取到总RNA，但是...
TRIM5α(tripartite motif protein 5-alpha)蛋白是恒河猴体内一种非常重要的限制因子，能抑制人免疫缺陷病毒(HIV-1, human immunodeficiency virus type 1)、马感染性贫血病毒(EIAV, equine infectious anemia virus)和猫免疫缺陷病毒 (FIV, feline immunodeficiencyv...
拟南芥中1个新snoRNA基因的鉴定
拟南芥 snoRNA AthZ270
2009/12/23
［目的］ 鉴定拟南芥中1个新snoRNA基因。［方法］ 运用生物信息学方法筛选拟南芥基因组序列，并对候选的基因序列结构、基因组织形式和功能进行分析。［结果］ 在拟南芥基因组中发现snR95 box H/ACA snoRNA上游有一段序列具有典型box C/D snoRNA的保守组件和结构特征，并具有2段与rRNA互补、长度超过数10个核苷酸的反义序列，该基因下游的反义序列与snoRNA数据库中...
DNA拓扑异构酶Ⅱ与Ⅰ A的进化分析
DNA拓扑异构酶 CAP-like结构域 Toprim结构域 分子进化 催化机制
2009/6/25
DNA拓扑异构酶与断开DNA链和改变其拓扑结构有关。先前的研究认为，拓扑异构酶Type ⅡB是由Type ⅡA通过基因的复制、重组和缺失所造成。然而，本研究结果则显示，Type ⅠA与Type ⅡB的进化关系较近，而Type ⅡA与Type ⅡB的关系较远。因此，Type ⅡB可能是由Type ⅠA演化而来，或者说，Type ⅡB是Type ⅠA在古细菌中的特化形式；Type ⅡB可能是由Type...
miR-34基因家族的分子进化
microRNA miR-34 分子进化
2009/6/25
根据miRNA基因在进化中高度保守的特点，利用生物信息学方法在目前已测序的动物物种中搜寻参与哺乳动物早期发育调控的mir-34基因的同源序列，在33个不同的动物物种中获得了miR-34基因的54条同源序列，其中18条为新发现的序列。表明miR-34是高度保守的，广泛存在于后生动物中。目前发现的mir-34基因80％位于基因间隔区，少数位于蛋白编码基因的内含子区和3′UTR上。不同动物中，mir-3...
在pH 7.4 的Tris-HCl的缓冲溶液中，采用紫外及荧光光谱法研究了荷叶中紫云英苷(AST)与DNA之间的相互作用，探讨了离子强度和阴离子猝灭剂KI对紫云英苷及紫云英苷-DNA体系荧光强度的影响，同时考察了紫云英苷和中性红与DNA结合的竞争性。结果表明DNA通过静态猝灭作用机制猝灭紫云英苷的荧光，并测得其在298及308 K时的猝灭速率常数(Kq)分别为3.120 ×1012和2.630×1...
Ce离子对寡聚脱氧核苷酸的断裂作用
寡聚脱氧核苷酸 Ce离子
2009/1/12
报道了Ｃｅ离子对人工设计合成的２６ｂｐ的单链ＯｌｉｇｏＤＮＡ的水解断裂作用．Ｃｅ（Ⅲ）需要有氧气存在的条件下，氧化生成Ｃｅ（Ⅳ）后才能切断ＯｌｉｇｏＤＮＡ，系统研究了各种条件下Ｃｅ（Ⅳ）对ＯｌｉｇｏＤＮＡ的切断反应，在酸性、碱性条件下都能水解切断ＯｌｉｇｏＤＮＡ，在近中性时切断作用最强。随着反应温度的升高、浓度的增大大反应时间的延长，切断反应越来越明显，并提出了反应机理，建立了用变性聚丙烯酰胺胺凝...