搜索结果: 16-30 共查到“知识库 动物遗传学”相关记录1191条 . 查询时间(0.583 秒)
拟通过比较杜洛克公猪与陆川母猪的二元杂交猪（简称杜陆猪，DLC）和杜洛克公猪与隆陆母猪（隆林公猪与陆川母猪的后代）的三元杂交猪（简称杜隆陆猪，DLLC）在生长、胴体和肉质性状上的表现来证实三元杂交的可行性。本研究分别屠宰了7月龄左右、约75 kg的DLC猪（295头）和DLLC猪（91头），测定其胴体直长、胴体斜长、背膘厚和眼肌面积等胴体性状以及肉色、pH、滴水损失、大理石纹和肌内脂肪含量等肉质性...
旨在调查恩施黑猪基因组群体遗传学参数与选择信号。本研究利用Porcine 80K SNP芯片，通过计算亲缘系数、近交系数、连锁不平衡程度与有效群体大小等群体遗传学层面的参数评估恩施黑猪种群结构关系，运用CLR和iHS方法检测恩施黑猪基因组选择信号，并通过生物信息学分析揭示其潜在受选择基因。亲缘系数计算发现，咸丰县恩施黑猪个体间平均亲缘系数为0.12；近交系数计算表明，16%的样本近交系数大于0.1...
旨在分析成年健康牦牛和犏牛（3~4岁）睾丸组织中长链非编码RNA（long non-coding RNA，lncRNA）的表达谱，从而探究lncRNA与犏牛雄性不育机制的关联性，为解释犏牛雄性不育的机理提供理论依据。采集成年（3~4岁）健康牦牛、犏牛各3头的睾丸组织，构建cDNA文库后进行高通量测序，筛选出差异的lncRNA，通过对差异显著的lncRNA与mRNA位置关系以及结合能的判定预测顺式作...
为探讨水体铜（Cu）和镉（Cd）污染对草鱼免疫系统的影响，本研究利用实时荧光定量PCR(QRTPCR)技术，分析在不同时间(2、4、6、8 d)及不同质量浓度的Cu2+(0.10、0.20、0.40、0.60、0.80 mg/L)和Cd2+(0.05、0.10、0.20、0.30、0.40 mg/L)暴露下，草鱼肾脏中3种白细胞介素——白细胞介素-1β、白细胞介素-8和白细胞介素-10基因表达量...
泥东风螺线粒体基因组及其遗传多样性分析
泥东风螺 线粒体基因组 环形结构 遗传多样性
2019/1/26
为研究泥东风螺线粒体基因组遗传进化和遗传多样性，采用PCR扩增和直接测序技术，对泥东风螺线粒体基因组全序列及3个野生群体线粒体基因测序，并用生物软件进行分析。研究结果表明，泥东风螺线粒体基因组上的tRNA-Met、tRNA-Tyr、tRNA-Cys、tRNA-Trp、tRNA-GLn、tRNA-Gly、tRNA-Glu和tRNA-Thr位于环形线粒体L链上；18种腹足纲动物线粒体全长基因相似性为7...
Genetic relationship between type traits, number of lactations initiated, and lifetime milk performance in Czech Fleckvieh cattle
dairy cows longevity lifetime performance conformation traits
2017/12/25
Genetic relationship was analyzed between type traits and longevity measures in dual-purpose cattle. Data from 91 486 Czech Fleckvieh cows first calved between 2003 and 2009 were used. Longevity was d...
选取捻转血矛线虫脂肪酶（Lipase）进行基因克隆、表达并对其生物学特性进行探究。PCR克隆捻转血矛线虫lipase基因，构建表达载体，获得融合蛋白质，采用Western blot检测该蛋白质的抗原特性；酯类酶解反应检测该融合蛋白质脂肪酶活性；实时定量PCR检测该基因在捻转血矛线虫不同发育阶段的转录情况；间接免疫荧光试验检验该抗原蛋白质在虫体组织的定位情况。结果表明，去除lipase基因信号肽序列...
贵妃鸡多趾性状遗传规律分析及多趾候选基因的表达
贵妃鸡 多趾性状 遗传规律 基因表达
2018/4/16
旨在探究贵妃鸡多趾性状的遗传规律和多趾候选基因在胚胎发育时期的表达，为贵妃鸡多趾品系的选育和多趾分子标记的开发奠定基础。选用不同趾型（四四趾、四五趾、五五趾）的贵妃鸡进行杂交和测交试验，统计分析后代各种趾型出现的比例，与理论值进行卡方检验。采取胚胎发育第5~10天的肢芽组织样品，测定分析多趾候选基因Lmbr1、SHH、RNF32、NOM1、MNX1、Gli3的表达量。结果表明，四五趾×四五趾组合多...
为探讨兔痒螨是否会受兔品种、温度带和地域来源的不同而出现遗传变异，采用PCR技术首次对我国6个省份共68个獭兔来源（陕西、河北、河南和湖北）和肉兔来源（山东和四川）痒螨虫株的线粒体细胞色素氧化酶b（cytb）基因全序列进行扩增，经测序后分析遗传变异情况。68条兔痒螨cytb全序列均为1 100 bp，共有96个（8.7%）变异位点和46个单倍型；我国整体种群的单倍型多样性（Hd）和核苷酸多样性（π...
Genetic relationship between type traits, number of lactations initiated, and lifetime milk performance in Czech Fleckvieh cattle
dairy cows longevity lifetime performance conformation traits genetic correlation
2018/3/5
Genetic relationship was analyzed between type traits and longevity measures in dual-purpose cattle. Data from 91 486 Czech Fleckvieh cows first calved between 2003 and 2009 were used. Longevity was d...
Repeats as global DNA methylation marker in bovine preimplantation embryos
epigenetics retrotransposon bisulfite sequencing
2017/2/21
DNA methylation undergoes dynamic changes and is a crucial part of the epigenetic regulation during mammalian early development. To determine the DNA methylation levels in bovine embryos, we applied a...
Dynamic expression of HSP90B1 mRNA in the hypothalamus of two Chinese chicken breeds under heat stress and association analysis with a SNP in Huainan chickens
heat shock protein mRNA level single nucleotide polymorphism thermal stress
2017/2/21
The effects of heat stress on HSP90B1 messenger RNA (mRNA) expression in the hypothalamus of chicken were investigated and HSP90B1 variations were detected. Females of two Chinese chicken breeds (Huai...
Association of missense MTTP gene polymorphism with carcass characteristics and meat quality traits in pigs
microsomal triglyceride transfer protein non-synonymous SNP pork quality
2017/2/21
Microsomal triglyceride transfer protein, coded by MTTP gene, has multiple functions including participation in formation of chylomicrons, low-density lipoproteins, and very low-density lipoproteins. ...
Random Regression Models Using Legendre Polynomials to Estimate Genetic Parameters for Test-day Milk Protein Yields in Iranian Holstein Dairy Cattle
Holstein Dairy Cattle Milk Protein Yields Random Regression Model Test-day Records
2016/12/28
The objective of this study was to estimate the genetic parameters of milk protein yields in Iranian Holstein dairy cattle. A total of 1,112,082 test-day milk protein yield records of 167,269 first la...