搜索结果: 121-135 共查到“知识库 动物遗传学”相关记录1191条 . 查询时间(0.999 秒)
中华菊头蝠MHCⅡ-DRB 基因遗传多态性及进化
中华菊头蝠 主要组织相容性复合体(MHC) DRB 遗传多样性
2016/7/19
采样自云南同一种群的中华菊头蝠共16 个个体,用于DRB 基因的分子进化和多态性研究。利用翼膜组织提取DNA 基因组,并PCR 克隆测序分析。获得了相差3 bp 的两种不同长度序列类型,A 序列类型263 bp,在研究群体中有15 个等位基因;B 序列类型260 bp,在研究群体中有8 个等位基因。在分析的74 个氨基酸变异位点上检测到12 个正向选择位点。在9 个个体中检测到分布频率最高的等位基...
动物microRNA-181的功能与应用前景
microRNA-181 细胞增殖 分化 免疫 肿瘤
2014/3/17
microRNA(miRNA)是一类短的、进化上高度保守的非蛋白编码RNA, 长度一般为17~25个核苷酸, 通过阻止靶mRNA的翻译或与之互补配对诱导靶基因降解来调控其表达。文章简要总结了microRNA-181(miR-181)在动物细胞增殖、凋亡和分化中的作用和调控机制, 探讨了miR-181对淋巴细胞的增生分化、自身免疫、炎症和抗病毒等方面的免疫调控作用, 并简要分析了miR-181在肿瘤...
猪AQP11基因微环表达载体的构建
猪 水通道蛋白11 微环表达载体
2014/3/18
旨在构建猪AQP11基因高效表达载体，观察该载体转染Hela细胞的转染效果。本研究通过PCR方法从猪肠道黏膜组织中克隆AQP11基因，构建了微环表达载体minicircle-AQP11，通过瞬时转染Hela细胞观察绿色荧光蛋白表达。结合测序结果表明，本研究成功地克隆了AQP11基因的全序列,包含了完整的CDS区，构建了AQP11基因的微环表达载体，在Hela细胞中观察到了持续1周的绿色荧光蛋白表达...
Genetic Diversity of mtDNA D-loop Polymorphisms in Laotian Native Fowl Populations
Native Fowl Genetic Variability mtDNA Maternal Origin
2016/5/16
Here, we studied the genetic diversity of native fowls in Laos by analyzing a mitochondrial DNA (mtDNA) sequence polymorphism. A 546-bp fragment of the mtDNA D-loop region was sequenced in 129 chicken...
Molecular Characterization and Expression Analysis of Ribosomal Protein S6 Gene in the Cashmere Goat (Capra hircus)
Ribosomal Protein (S6) Gene Expression Pattern The Mammalian Target of Rapamycin (mTOR)
2016/5/12
Ribosomal protein (rp) S6 is the substrate of ribosomal protein S6K (S6 kinase) and is involved in protein synthesis by mTOR/S6K/S6 signaling pathway. Some S6 cDNA have been cloned in mammals in recen...
Genome-wide Association Study of Chicken Plumage Pigmentation
Chicken Plumage Pigmentation Genome-wide Association Study
2016/5/12
To increase plumage color uniformity and understand the genetic background of Korean chickens, we performed a genome-wide association study of different plumage color in Korean native chickens. We ana...
The Possibility of TBC1D21 as a Candidate Gene for Teat Numbers in Pigs
QTL SNP SSC7 TBC1D21 Teat Number Pig
2016/5/12
Based on a quantitative traits locus (QTL) study using a F2 intercross between Landrace and Korean native pigs, a significant QTL affecting teat numbers in SSC7 was identified. The strong positional c...
Genetic Variation and Phylogenetic Relationships of Indian Buffaloes of Uttar Pradesh
Buffalo Mitochondrial DNA D-loop Region Neutrality Test Phylogenetic Analysis
2016/5/12
India possesses a total buffalo population of 105 million out of which 26.1% inhabit Uttar Pradesh. The buffalo of Uttar Pradesh are described as nondescript or local buffaloes. Currently, there is no...
Identification of Stearoyl-CoA Desaturase (SCD) Gene Interactions in Korean Native Cattle Based on the Multifactor-dimensionality Reduction Method
Native Cattle Multifactor-Dimensionality Reduction (MDR) Single Nucleotide Polymorphism (SNP) Stearoyl-CoA Desaturase1 (SCD1) Gene
2016/5/12
Fat quality is determined by the composition of fatty acids. Genetic relationships between this composition and single nucleotide polymorphisms (SNPs) in the stearoyl-CoA desaturase1 (SCD1) gene were ...
Gene Expression Profiling in the Pituitary Gland of Laying Period and Ceased Period Huoyan Geese
Suppression Subtractive Hybridization Quantitative Real-time PCR
2016/5/12
Huoyan goose is a Chinese local breed famous for its higher laying performance, but the problems of variety degeneration have emerged recently, especially a decrease in the number of eggs laid. In ord...
Development of Genetic Markers for Triploid Verification of the Pacific Oyster, Crassostrea gigas
Triploid Oyster Microsatellite Multiplex PCR
2016/5/12
The triploid Pacific oyster, which is produced by mating tetraploid and diploid oysters, is favored by the aquaculture industry because of its better flavor and firmer texture, particularly during the...
Genetic Polymorphisms of the Bovine NOV Gene Are Significantly Associated with Carcass Traits in Korean Cattle
Single Nucleotide Polymorphism Carcass Weight Back Fat Thickness Korean Cattle
2016/5/11
The objective of this study was to investigate single nucleotide polymorphisms (SNPs) in the bovine nephroblastoma overexpressed (NOV) gene and to evaluate whether these polymorphisms affect carcass t...
Presence of Transcription Factor OCT4 Limits Interferon-tau Expression during the Pre-attachment Period in Sheep
Interferon-tau Transcription Gene regulation Implantation Sheep
2016/5/11
Interferon-tau (IFNT) is thought to be the conceptus protein that signals maternal recognition of pregnancy in ruminants. We and others have observed that OCT4 expression persists in the trophectoderm...
Investigation of MC1R SNPs and Their Relationships with Plumage Colors in Korean Native Chicken
Korean Native Chicken MC1R Plumage Color SNPs
2016/5/11
The melanocortin 1 receptor (MC1R) gene is related to the plumage color variations in chicken. Initially, the MC1R gene from 30 individuals was sequenced and nine polymorphisms were obtained. Of these...
Microarray Analysis of Genes Involved with Shell Strength in Layer Shell Gland at the Early Stage of Active Calcification
Chicken Microarray Differentially Expressed Genes Eggshell Strength
2016/5/11
The objective of this study was to get a comprehensive understanding of how genes in chicken shell gland modulate eggshell strength at the early stage of active calcification. Four 32-week old of pure...