搜索结果: 61-75 共查到“知识库 动物病毒学”相关记录81条 . 查询时间(3.033 秒)
SARS冠状病毒S蛋白结构预测及表达初探
SARS 冠状病毒 S蛋白 表达
2008/2/1
摘要利用生物信息学的工具和方法，对SARS病毒基因组中预测的S蛋白（spike protein）的编码序列进行了分析，预测了其结构特征和可能的功能域。选择其中最有可能参与受体识别及产生主要免疫原性的区域(S401～659)作为待表达区域。将通过PCR获得的此段S蛋白片段的编码序列克隆至大肠杆菌载体pET28a(+)和酵母表达载体pPIC9K中，分别构建了pET28a(+)-S和pPIC9K-S表达...
病毒感染和染色体畸变
2008/1/21
病毒广泛分布于人类环境之中，人类病毒性疾病种类繁多，流行又广。自Hampar等(1961) 在单纯疱疹病毒感染的中国地鼠细胞中，首次发现染色体畸变以来，大量的工作相继证明，在许多病毒性疾病患者以及离体培养的人类细胞中，病毒染有着强烈的细胞遗传学效应即诱发人类染色体畸变。在某种意义上，我们可以把病毒称为拟辐射因子，而且可能是人类环境中最普遍的一种诱变因子。’在本文中，我们将讨论病毒作为一种强烈的生物...
猪Ⅱ型圆环病毒ORF1基因克隆及在E.coli中的表达 。
表达猪繁殖与呼吸综合征病毒GP3基因的重组伪狂犬病病毒的构建
porcine reproductive and respiratory syndrome virus pseudorabies virus transfer vector
2007/12/20
Based on the nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) CH 1a strain, a pair of primers was designed. The GP3 gene of PRRSV HB 1 strain was cloned by RT PCR. Th...
根据GenBank发布的猪2型圆环病毒(PCV2)序列(AY035820)，设计两对特异性引物，采用PCR方法，分别扩增了猪2 型圆环病毒ORF1和ORF2基因。将ORF1和ORF2基因的PCR产物回收并酶切后，依次插入到伪狂犬病毒gE／gI双缺失通用转移栽体DIECMV中，构建了猪2型圆环病毒．伪狂犬病毒重组中问转移质粒pIEORF1一ORF2。采用脂质体介导法，将重组中间转移质粒pIEORF1...
SARS病毒传播与气象条件有关
2007/8/15
日前，中国气象局国家气候中心的科研人员通过研究得出结论，认为SARS病毒传播与气象条件有关系。
具体研究成果为：空气的最高温度、气温日较差和相对湿度等气象要素与SARS病毒的传播有着密切关系。在病毒暴发前9—10天日最高温度相对较低(26摄氏度以下)、气温日较差较小、空气相对湿度较大的情况下，有利于SARS病毒扩散和传播；反之，不利于SARS病毒的扩散和传播。此研究结论所依据的主要为北京地区的数据...
题目：茶尺蠖小RNA病毒5'端非编码区的克隆和测序及与哺乳动物小RNA病毒的比较分析
作者：王小纯1,2， 张珈敏1， 蒋洪1， 俞海洋1， 谭莉1， 胡远扬1*
摘要：用Trizol从纯化的茶尺蠖Ectropis oblique小RNA病毒（EoPV）中提取病毒基因组RNA，逆转录后加poly(dT)，然后进行两步PCR扩增基因组5′端。克隆测序后，对其5′端非编码区的核苷酸序列进行分析，发现...
Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture
Retrovirus Gene Transfer Electroporation Stem Cell Medium
2016/4/26
The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be re...
Insertional Variations of Two Porcine Endogenous Retroviruses (PERVs) in Korean Native Pigs and Asian Wild Boars
Pigs Insertional Variations Porcine Endogenous Retroviruses (PERVs) Xenotransplantation
2016/4/25
Porcine Endogenous Retroviruses (PERVs) are a major concern in relation to xenotransplantation. Previous research indicated that PERVs are present at about 50 copies in the pig genome and their chromo...
Effects of Gal-13 on the Content of Immunoglobulin, Proliferation of Lymphocyte and Antibody Titers after Vaccination with Infectious Bursal Disease Virus Vaccine in Chickens
Gal-13 Antimicrobial Peptides Chicken Intestine Adaptive Immunity
2016/4/25
Gal-13 is an antimicrobial peptide isolated from chicken intestine. Ninety chickens were randomly divided into two groups (45 chickens for each group) to determine the effect of oral administration of...
Prevalence of Lymphocyte Nuclear Pockets in Holstein-Friesian Dairy Cattle Infected with Bovine Leukemia Virus in Korea
BLV EBL Bovine Enzootic Pocket Leukemia
2016/4/19
The integral relationship between the occurrence of lymphocyte nuclear pockets (LNPs) and BLV-infection was examined in Holstein-Friesian dairy cattle in Korea. Transmission electron microscope (TEM) ...
Characteristics of Bovine Lymphoma Caused by Bovine Leukemia Virus Infection in Holstein-Friesian Dairy Cattle in Korea
BLV EBL Bovine Enzootic Gross Finding Leukemia
2016/4/19
The frequency and distribution of lymphoma caused by bovine leukemia virus (BLV) infection in various organs were investigated. Lymphoma samples were obtained from slaughtered cattle or from cattle su...
Release of Newcastle Disease Virus Vaccine from Chitosan Microspheres In vitro and In vivo
Newcastle Vaccine Chitosan Microspheres Macrophage Interleukin-1
2016/4/15
Newcastle disease vaccine (NDV)-loaded chitosan microspheres (NDV-CM) were prepared. Stimulatory effects of these NDV-CM on antibody response compared to free NDV were examined in vitro and in vivo. I...
Germ-line Transmission of Pseudotyped Retroviral Vector in Chicken
Retroviral Vector Transgenic Chicken Embryos Germ Line Transmission
2016/4/14
Using MLV (murine leukemia virus)-based retrovirus vectors encapsidated with VSV-G (vesicular stomatitis virus G glycoprotein), we tried to make transgenic chickens carrying the transferred genes in t...
禽流感的发生及其防治
禽流感防治 野生鸟类保护
2013/7/9
依据禽流感病毒的多样性、高变异性以及野生鸟类自然储存库等特点，结合我国地理、生态和养禽业的具体情况，对目前我国爆发的禽流感提出一些观点性建议。