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Regulation of glucose homeostasis by Doc2b and Munc18 proteins.
Diabetes SNARE proteins exocytosis
2015/5/25
Glucose homeostasis is maintained through the coordinated actions of insulin secretion from pancreatic beta cells and insulin action in peripheral tissues. Dysfunction of insulin action yields insulin...
Identification and characterization of altered mitochondrial protein acetylation in Friedreich's ataxia cardiomyopathy
Identification and characterization mitochondrial protein acetylation
2015/5/21
Friedreich’s Ataxia (FRDA) is a rare and poorly understood autosomal recessive disease caused by a pathological deficiency of the mitochondrial protein frataxin. Patients suffer neurodegeneration, ata...
通过研究Lactobacillus delbrueckii QS306在10%的还原脱脂牛乳中的生长特性,探讨培养基中添加核酸关联物质时对蛋白质水解能力及其水解产物对血管紧张素转化酶(ACE)抑制活性的影响。结果表明,菌株QS306在10%的还原脱脂牛乳中生长缓慢,产酸量和生物量逐渐上升,在37℃培养第4 d时,滴定酸度和生物量分别达到68.24(°T)和6.91(OD410 nm×10),蛋白质...
Resistance in carps (Cyprinus carpio L.) affected by a natural bacterial infection
myeloperoxidase serum proteins sick carps
2015/1/20
Sick carps, affected by acute or chronic forms of erythrodermatitis (CE) were studied. The studies were aimed at obtaining pathophysiological data which would permit evaluation of clinical status and ...
乙酰化改性对米渣蛋白功能性质的影响
乙酰化改性 米渣蛋白 功能性质
2016/5/27
采用乙酰化对米渣蛋白进行改性,研究了米渣蛋白在乙酰化改性过程中巯基和二硫键含量、表面疏水性、溶解性以及乳化性的变化。结果表明,随改性程度的增加,米渣蛋白中暴露巯基含量逐渐增加,总游离巯基含量逐渐下降,二硫键含量逐渐增加,表面疏水性先下降后升高。乙酰化改性后的米渣蛋白在p H5~10范围内的溶解性、乳化活性和乳化稳定性明显增加,但是p H2~4范围内的溶解性和乳化活性却有所降低。可见,乙酰化改性能改...
本文纯化了一种小麦麸皮抗冻蛋白,并对其氨基酸组成、二级结构进行了分析。采用粗分离、饱和硫酸铵沉淀、离子交换色谱(DEAE-cellulose-26)、凝胶过滤色谱(Sephadex G75)四步对其进行纯化,纯化倍数为449.99倍,产率为4.17%,分子量为11.2 ku,热滞活性为0.169℃(5 mg/m L)。氨基酸组成结果表明:其甘氨酸含量较高(52.08 mol%),亲水性好。将其与几...
MoRFs A Dataset of Molecular Recognition Features
protein sequences Molecular Recognition Features Molecular Recognition Elements protein-protein interactions intrinsic protein disorder bioinformatics
2015/1/9
The last decade has witnessed numerous proteomic studies which have predicted and successfully confirmed the existence of extended structurally flexible regions in protein molecules. Parallel to these...
Conversation of Intrinsic Disorder in Protein Domains and Families
disorder protein domains
2015/1/9
Protein regions which lack a fixed structure are called ‘disordered’. These intrinsically disordered regions are not only very common in many proteins, they are also crucial to the function of many pr...
A review of factors affecting the success of membrane protein crystallization using bicelles
Biochemistry bicelle membrane protein crystallization
2015/1/7
Several reports have been published detailing various platforms for obtaining crystals of membrane proteins to determine their structure including those that use disk shaped bilayers called bicelles. ...
丙二醛氧化对大米蛋白功能性质的影响
大米蛋白 丙二醛 蛋白质氧化 功能性质
2016/6/28
以新收获籼米为原料制备大米蛋白,采用不同浓度丙二醛氧化大米蛋白,研究丙二醛氧化对大米蛋白功能性质的影响。结果表明:随着丙二醛浓度的增加,氧化大米蛋白的羰基和二硫键含量增加,游离巯基含量下降,表明大米蛋白发生了氧化。当丙二醛浓度从0增加到100 mmol/L时,氧化大米蛋白溶解性从28.88%降低至12.20%,持水性从353.67%降低至132.33%,持油性从89.40%上升至189.40%,起...
Analyses of Arabidopsis Yellow Stripe-Like (YSL) Family of Metal Transporters
Iron uptake Yellow stripe-like proteins
2015/1/5
Iron is one of the most important micronutrients used by living organisms. Iron is frequently a limiting nutrient for plant growth, and plants are a major source of iron for human nutrition. The most ...
Properties of Potential Substrates of a Cyanobacterial Small Heat Shock Protein
small heat shock protein substrates Synechocystis heat stress
2014/12/12
Most proteins must fold into native three-dimensional structures to be functional. But, newly synthesized proteins are at high risk of misfolding and aggregating in the cell. Stress, disease or mutati...
Human Niemann-Pick Type C2 Disease Protein Expression, Purification and Crystallization
Niemann-Pick type C2 NPC2 Structure Expression Crystallization Purification
2014/12/12
Niemann-Pick type C2 (NPC2) protein is a soluble protein that binds unesterified cholesterol. The protein helps transport unesterified cholesterol in tandem with the membrane protein Niemann-Pick type...
Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the NUM1 Coiled Coil Domain
Connecting Motors and Membranes Dynein Pathway Components vitro Characterization NUM1 Coiled Coil Domain
2014/12/12
In the budding yeast, Saccharomyces Cerevisiae, dynein, a minus-end directed motor, is involved in nuclear migration and proper orientation of the mitotic spindle during mitosis. Our lab has developed...
In Vivo Labeling Of A Model β-Clam Protein With A Fluorescent Amino Acid
nonsense suppression technique L-(7-hydroxycoumarin-4-yl) ethyl glycine unnatural amino acid CRABP I
2014/12/12
Proteins can be labeled with different tags to enable their structural and functional investigations. In addition, labeling proteins at specific sites helps in studying the conformational dynamics of ...