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Amplification of Porcine SRY Gene for Sex Determination
SRY Gene Sex Determination Sperm Sorting Duplex PCR
2016/4/29
The separation of X and Y chromosome-bearing sperm is of use in many aspects of livestock maintenance. In this study, we sought to determine the difference in DNA content between X- and Y-bearing sper...
摘要应用人的性别决定基因SRY(Sex-determining Region Y gene,SRY)中HMG框内的一对引物，对小麂细胞株的基因组DNA进行PCR扩增，得到雄性小麂细胞的220bp扩增产物，而在雌性小麂细胞中未发现扩增产物。将雄性小麂细胞的220bp扩增产物通过T-A互补法克隆到质粒pGEM-T 载体中，筛选阳性克隆进行DNA测序。测序结果表明小麂Sry基因保守序列与人的SRY基因保...
A Y-linked SNP in SRY Gene Differentiates Chinese Indigenous Swamp Buffalo and Introduced River Buffalo
Swamp buffalo River buffalo SRY SNP Genetic diversity Conservation
2016/4/21
The complete coding region sequence of the SRY gene in Chinese swamp buffalo was determined by PCR product sequencing. Comparison of swamp and river buffalo SRY gene sequences revealed a single nucleo...