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Localization of the L-selectin (CD62L) and β2-integrin (CD18) bearing cells in different tissues of the bovine mammary gland was examined. Five dairy cows of Holstein-Friesian breed in the middle of t...
Breast cancer is the most frequent tumor type among women. Heightened susceptibility of the breast to tumor development has been associated with early menarche, nulliparity, exposures to ionizing radi...
Distribution of leukocyte types present in virgin bovine mammary glands was analysed in dot plots obtained by flow cytometry (FACS) of samples collected from 10 non-pregnant heifers after induction of...
The regulation of genes involved in proliferation and cell cycle control plays a critical role in normal development and differentiation. Aberrant expression of genes promoting proliferation (oncogene...
Ten, first lactation, 87.5%HF dairy cattle were used to investigate effects of long-term administration of recombinant bovine somatotropin (rbST) on nutrient uptake by the mammary gland at different s...
摘要 为探讨应用牛催乳素促进乳腺生物反应器高效表达外源蛋白(如人乳铁蛋白、人血小板生成素)的可行性,构建两种牛催乳素和外源基因乳腺共表达载体。一种为牛催乳素基因和外源基因共用一个6.7 kb山羊β-酪蛋白启动子启动转录,两个基因以内部核糖体进入位点(IRES)连接;另一种为外源基因利用6.7 kb山羊β-酪蛋白启动子,催乳素基因利用2.0 kb山羊β-酪蛋白启动子,分别转录。脂质体转染后,RT...
摘要通过长距离PCR从山羊基因组DNA分两段扩增山羊β乳球蛋白(β-lactoglobulin,BLG)基因,扩增出的两个片段分别克隆到T载体上,利用BLG基因序列自身存在的NarI单酶切位点进行拼接,获得了全长为7.2kb的山羊BLG基因克隆,并构建了它的真核表达载体,经酶切鉴定和序列分析证实了克隆的正确性。用线性化的BLG基因显微注射小鼠受精卵以建立转基因鼠,经PCR和Southern印迹分析...
Ten, first lactation, 87.5%HF dairy cattle were used to investigate effects of long-term administration of recombinant bovine somatotropin (rbST) on nutrient uptake by the mammary gland at different s...
In our previous study, overexpression of extracellular proteinase inhibitor (Expi) gene accelerated apoptosis of mammary epithelial cells, and induced expression of B cell activating factor (BAFF) gen...
Physiological parameters were measured on six primiparous, non-pregnant Holstein cows prior to peak lactation over a 3-month summer season in southwestern Taiwan. The objectives were to characterize h...
The objective of this study was to examine the effect of compensatory growth nutritional regimen on mammary gland growth and lactation. One hundred twenty-two Sprague Dawley female rats (35 days of ag...
To understand molecular mechanisms involved in bovine mammary gland growth, expression of stat5a gene was examined in bovine mammary tissues. We found that stat5a gene was highly induced at pregnant 7...
Effects of active immunization against native 14-mer somatostatin (SRIF, somatotropin releasing inhibiting factor) and its two 14-mer-somatostatin analogues on the milk production in rat mammary cells...

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