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中国科学院的新型基因编辑工具脱靶检测方法GOAT证明CRISPR-Cas12a不存在明显的脱靶效应（图）
新型 基因编辑 脱靶检测方法 GOAT证明 CRISPR-Cas12a 脱靶效应
2021/4/7
2021年4月2日，《Protein & Cell》发表了一篇名为《Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos》的研究论文，该研究由中科院脑科学与智能技术卓越创新中心（神经科学研究所）、上海脑科学与类脑研究中心周昌阳...
Molecular characterization and expression pattern of a novel Keratin-associated protein 11.1 gene in the Liaoning cashmere goat (Capra hircus)
Liaoning Cashmere Goat Keratin-associated Protein 11.1 (KAP11.1) Expression Pattern
2017/3/9
An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness.
Effects of Weaning and Spatial Enrichment on Behavior of Turkish Saanen Goat Kids
Environmental Enrichment Turkish Saanen Growth Bipedal Animal Welfare
2016/7/27
As is in all economic activities, the highest yield per unit area is the main goal in animal production, while addressing the temperamental needs of animals often is ignored. Animal welfare is not onl...
Characteristics and Gel Properties of Gelatin from Goat Skin as Influenced by Alkaline-pretreatment Conditions
Goat Gelatin Skin Pretreatment Alkaline Gel Strength
2016/7/27
Characteristics and properties of gelatin from goat skin pretreated with NaOH solutions (0.50 and 0.75 M) for various times (1 to 4 days) were investigated. All gelatins contained α-chains as the pred...
Cloning and Characterization of an Endoglucanase Gene from Actinomyces sp. Korean Native Goat 40
Korean Native Goat Actinomyces sp Endo-β-1 4-glucanase Cellulase
2016/5/20
A gene from Actinomyces sp. Korean native goat (KNG) 40 that encodes an endo-β-1,4-glucanase, EG1, was cloned and expressed in Escherichia coli (E. coli) DH5α. Recombinant plasmid DNA from a positive ...
Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat
Boer goat collection techniques oocyte maturation ovary medium
2015/6/4
The oocytes (experiment 1) were harvested by one of the four collection techniques (slicing, puncture, aspiration I and aspiration II) and the total number and the number of each grade of oocytes were...
An MspI PCR-RFLP detecting a single nucleotide polymorphism at alpha-lactalbumin gene in goat
goat alpha-lactalbumin gene polymorphism Single Nucleotide Polymorphism (SNP) PCR-RFLP
2015/6/4
Alpha-lactalbumin (α-LA, LALBA) was strongly correlated with the nutritional value and the functional properties of whey and whey products. However, there are not many studies of goat LALBA gene and i...
SNP Discovery from Transcriptome of Cashmere Goat Skin
Transcriptome Goats Capra hircus RNA Sequencing
2016/5/18
The goat Capra hircus is one of several economically important livestock in China. Advances in molecular genetics have led to the identification of several single nucleotide variation markers associat...
Direct Interaction between Ras Homolog Enriched in Brain and FK506 Binding Protein 38 in Cashmere Goat Fetal Fibroblast Cells
mammalian Target of Rapamycin [mTOR] Ras homolog enriched in brain [Rheb]
2016/5/17
Ras homolog enriched in brain (Rheb) and FK506 binding protein 38 (FKBP38) are two important regulatory proteins in the mammalian target of rapamycin (mTOR) pathway. There are contradictory data on th...
Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
Goat Vascular Endothelial Growth Factor (VEGF) Recombinant Protein Hair Growth
2016/5/17
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic ex...
Dairy Goat Production
Dairy Goat Production
2013/6/28
Dairy goat production is an alternative livestock enterprise suitable for many small-scale or part-time livestock opera-tions. Some dairy goat producers have been successful in pasteurizing goat milk ...
Meat Goat Production
Meat Goat Production
2013/6/28
Goat meat is consumed widely throughout the world, es-pecially in developing countries. However, it is not usually sold in traditional marketplaces in the United States. Sheep and goat meat is the fou...
Reference Gene Screening for Analyzing Gene Expression Across Goat Tissue
Reference Gene Expression Stability Tissue Goat
2016/5/12
Real-time quantitative PCR (qRT-PCR) is one of the important methods for investigating the changes in mRNA expression levels in cells and tissues. Selection of the proper reference genes is very impor...
Molecular Characterization and Expression Analysis of Ribosomal Protein S6 Gene in the Cashmere Goat (Capra hircus)
Ribosomal Protein (S6) Gene Expression Pattern The Mammalian Target of Rapamycin (mTOR)
2016/5/12
Ribosomal protein (rp) S6 is the substrate of ribosomal protein S6K (S6 kinase) and is involved in protein synthesis by mTOR/S6K/S6 signaling pathway. Some S6 cDNA have been cloned in mammals in recen...
Metagenome Analysis of Protein Domain Collocation within Cellulase Genes of Goat Rumen Microbes
Goat Rumen Shot-gun Sequencing Metagenome Protein Domain
2016/5/12
In this study, protein domains with cellulase activity in goat rumen microbes were investigated using metagenomic and bioinformatic analyses. After the complete genome of goat rumen microbes was obtai...