搜索结果: 1-7 共查到“多肽与蛋白质生物化学 pH”相关记录7条 . 查询时间(0.109 秒)
美国肯塔基大学熊幼翎教授、江南大学蒋将教授:作为液体鸡蛋替代品的绿豆蛋白乳液的结构和流变特性:pH(图)
美国肯塔基大学 熊幼翎 江南大学 蒋将 液体鸡蛋 绿豆蛋白乳液 食品蛋白质化学 蛋白乳液
2022/4/29
浙江大学医学院段树民院士团队Cell Research发文报道溶酶体蛋白酶cathepsin D具有pH及构象依赖的双重酶活性(图)
浙江大学医学院 段树民院士 Cell Research 溶酶体蛋白酶 cathepsin D pH 构象依赖 双重酶活性
2021/2/4
溶酶体中存在多种负责降解多肽或蛋白质的蛋白水解酶。为确保它们在特定部位与环境发挥催化作用、避免“滥杀无辜”,蛋白水解酶通常以酶原的形式在囊泡内大量储存。在传统认识中,酶原是不具有催化活性的前体蛋白。然而,酶原在功能上真的只是“沉默的大多数”吗?
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.3.Temperature Dependence of Proton Transfer
Green Fluorescent Proteins Amino Acid Substitution Spectrometry, Fluorescence Kinetics Mutagenesis,Site-Directed Models,Theoretical Genetic Variation Hydrogen-Ion Concentration Thermodynamics Solvents
2016/5/24
In parts 1 and 2 of this series [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S. Y., Xi, L. X., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Bio...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.2.Excited-State Dynamics
Animals Protons Deuterium Luminescent Proteins Green Fluorescent Proteins Spectrometry, Fluorescence Biosensing Techniques Kinetics Genetic Variation Hydrogen-Ion Concentration
2016/5/24
In the preceding paper [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S., Xi, L., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Biochemistry 41, 1...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.1.Structural Characterization and Preliminary Application
Animals Cricetulus Cell Line Threonine Luminescent Proteins Green Fluorescent Proteins Microscopy, Confocal Crystallography, X-Ray Transfection Amino Acid Substitution
2016/5/24
Novel dual emission, pH-sensitive variants of the green fluorescent protein (GFP) have been constructed and are suitable for ratiometric emission measurements in vivo. This new class of GFPs, termed d...
固定pH梯度等电聚焦法分离纯化人胸腺活性肽
人胸腺肽 固定pH梯度等电聚焦 活性玫瑰花结法 末端转脱氧核苷酰酶
2007/12/21
摘要 本文用4—7月龄胎儿胸腺,按照匀浆—80℃加热—丙酮沉淀—抽提—超滤等一系列步骤制备人胸腺混合肽。用固定pH梯度聚丙烯酰胺凝胶等电聚焦技术,结合紫外薄层扫描法定位,从胸腺混合肽中得到四种纯化的胸腺肽HTP α_1,β_1,β_2和γ_1;分别测得其等电点为4.6,5.3,5.8和7.6,分子量分别为4800,6700,7200—7300和12,000—13,000。另外至少还得到四种部份纯化...