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Alternative splicing of MDM2 during breast tumorigenesis and mammary gland *development
Genes proliferation cell cycle growth abnormal genes oncogenes and tumor suppressor protein and tumor
2014/12/11
The regulation of genes involved in proliferation and cell cycle control plays a critical role in normal development and differentiation. Aberrant expression of genes promoting proliferation (oncogene...
催乳素和外源基因乳腺共表达载体可提高外源基因在哺乳细胞的表达Mammary Gland Dual-Expression Construct Containing Prolactin and Foreign DNAs Can Promote the Expression of Foreign Proteins in Mammary Cells
催乳素 乳铁蛋白 血小板生成素 表达
2008/1/8
摘要
为探讨应用牛催乳素促进乳腺生物反应器高效表达外源蛋白(如人乳铁蛋白、人血小板生成素)的可行性,构建两种牛催乳素和外源基因乳腺共表达载体。一种为牛催乳素基因和外源基因共用一个6.7 kb山羊β-酪蛋白启动子启动转录,两个基因以内部核糖体进入位点(IRES)连接;另一种为外源基因利用6.7 kb山羊β-酪蛋白启动子,催乳素基因利用2.0 kb山羊β-酪蛋白启动子,分别转录。脂质体转染后,RT...
摘要通过长距离PCR从山羊基因组DNA分两段扩增山羊β乳球蛋白(β-lactoglobulin,BLG)基因,扩增出的两个片段分别克隆到T载体上,利用BLG基因序列自身存在的NarI单酶切位点进行拼接,获得了全长为7.2kb的山羊BLG基因克隆,并构建了它的真核表达载体,经酶切鉴定和序列分析证实了克隆的正确性。用线性化的BLG基因显微注射小鼠受精卵以建立转基因鼠,经PCR和Southern印迹分析...