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In vitro Development of Interspecies Somatic Cell Nuclear Transfer Embryos Derived from Murine Embryonic Fibroblasts and Bovine Oocytes
Murine Bovine Interspecies Somatic Cell Nuclear Transfer b-Mercaptoethanol Xist
2016/4/28
Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studie...
Effects of Cell Status of Bovine Oviduct Epithelial Cell (BOEC) on the Development of Bovine IVM/IVF Embryos and Gene Expression in the BOEC Used or Not Used for the Embryo Culture
Bovine Oviduct Epithelial Cell Bovine IVM/IVF Embryos Growth Factor Gene Apoptosis Gene Antioxidant Gene
2016/4/28
The objective of this study was to investigate the effects of cell status of BOEC on development of bovine IVM/IVF embryos and gene expression in BOEC before or after culturing of embryos. The develop...
Histological Changes of Tissues and Cell Wall of Rice Straw Influenced by Chemical Pretreatments
Chemical Treatment Electron Microscope Histological Changes Rice Straw Stem Rumen Degradability
2016/4/27
Sodium hydroxide (SH) or ammonium bicarbonate (AB) were applied to rice straw to investigate the effects on histological change of stem tissue or cell wall before and after in sacco degradation using ...
Factors Influencing Satellite Cell Activity during Skeletal Muscle Development in Avian and Mammalian Species
Muscle Regeneration Muscle Development Mitogenic Factors Meat Production Poultry Mammals
2016/4/27
Avian and mammalian skeletal muscles exhibit a remarkable ability to adjust to physiological stressors induced by growth, exercise, injury and disease. The process of muscle recovery following injury ...
Ruminal Protein Degradation Characteristics of Cell Mass from Lysine Production
Cell Mass from Lysine Production Ruminal Protein Degradability Protein Fraction
2016/4/27
Chemical analysis and in vitro studies were conducted to investigate the nutritive value for ruminants of cell mass from lysine production (CMLP) which is a by-product of the lysine manufacturing proc...
植物程序性细胞死亡检测技术A Review of Methods for Measuring Plant Programmed Cell Death (PCD)
植物 程序性细胞死亡(PCD) 检测方法
2008/1/10
摘要 程序性细胞死亡(PCD)是细胞死亡的方式之一,在植物发育及逆境响应等方面起着重要作用。主要介绍检测植物PCD的细胞学、生物化学、分子生物学及生理学方法,以及流式细胞仪在植物PCD检测中的应用。
Abstract:Programmed cell death (PCD) is an active way for plant cells marching to death,which plays...
鉴定痢疾杆菌毒力基因的SW480细胞模型构建Construction of SW480 Cell Model Identifying Shigella Virulent Genes
SW480 细胞模型 痢疾杆菌 信号标签诱变技术 构建
2008/1/8
摘要
信号标签诱变技术(STM)是一种在体内高通量筛选病原体毒力基因的新方法,在应用时的一个先决条件是要建立合适的体内筛选系统。为将该技术应用于福氏痢疾杆菌,我们使用三个福氏痢疾杆菌菌株进行了预试验:通过同源重组构建而成的带有氯霉素抗性且aroA和virG基因失活的突变株RC426;因在侵袭质粒上自发缺失3个基因座(ipaBCDA, invA 和 virG)的另一减毒突变株T32,其曾被用作福氏...
缘悭一面廿五年――纪念我所在Cell杂志发表论文25周年
神经细胞 蛋白质 微生物
2007/12/29
2005年1月14日出版的美国生命科学权威杂志《细胞》发表了中国科学家(蒋辉、郭纬、梁新华和饶毅)关于发现对确定神经细胞极性起关键作用的蛋白质的研究论文。国内多家媒体报道,这是近25年来中国内地学者第一次在《细胞》杂志上发表论文。
Nitrogen Utilization of Cell Mass from Lysine Production in Goats
Cell Mass from Lysine Production Nitrogen Retention Rumen Fermentation Goat
2016/4/27
Two experiments were conducted to evaluate nutritive value of cell mass from lysine production (CMLP) as a protein supplement for ruminants. In each experiment, animals were fed a diet containing 40% ...
Somatic Cell Nuclear Transfer of Oocytes Aspirated from Postovulatory Ovarian Follicles of Superovulated Rabbits
Nuclear Transfer Oocyte Rabbit Follicle Somatic Cell
2016/4/26
The aim of this study was to evaluate if oocytes, aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration, could be efficiently used as ooplasm recipients ...
Effect of a Mixture of Conjugated Linoleic Acid (CLA) Isomers on T Cell Subpopulation and Responsiveness to Mitogen in Splenocytes of Male Broiler Chicks
CLA Broiler Chicks T Cell Population Splenocyte Proliferation
2016/4/26
The experiments were conducted to determine effects of a mixture of conjugated linoleic acid (CLA) isomers on T cell subpopulations and responsiveness to mitogen of splenocytes in male broiler chicks....
Steroid Effects on Cell Proliferation, Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells
Bovine Muscle Satellite Cell Steroid Primary Cell Culture
2016/4/25
The present study was conducted to establish primary bovine muscle satellite cell (MSC) culture conditions and to investigate the effects of various steroid hormones on transcription of the genes invo...
Production of Cloned Korean Native Goat (Capra hircus) by Somatic Cell Nuclear Transfer
Goat Superovulation Oocyte Cloning Nuclear Transfer Telomere
2016/4/25
The objectives of the present study were to initiate cloning of Korean native goat by somatic cell nuclear transfer (NT) and to examine whether unovulated (follicular) oocytes can support the same dev...
Expression of B Cell Activating Factor Pathway Genes in Mouse Mammary Gland
B Cell Activating Factor APRIL Apoptosis Mammary Gland
2016/4/25
In our previous study, overexpression of extracellular proteinase inhibitor (Expi) gene accelerated apoptosis of mammary epithelial cells, and induced expression of B cell activating factor (BAFF) gen...
Changes in Serum Biochemical Markers of Bone Cell Activity in Growing Thoroughbred Horses
Horses Biochemical Markers Of Bone Metabolism Body Weight Withers Height Growth
2016/4/22
We studied the changes in biochemical markers of bone metabolism in growing Thoroughbred horses. Serum osteocalcin (OC), as a marker for bone formation, and carboxy-terminal propeptide of type-I colla...