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Impact of polyunsaturated and saturated fat overfeeding on the DNA-methylation pattern in human adipose tissue: a randomized controlled trial
DNA methylation epigenetics EWAS Illumina 450k methylation array LIPOGAIN cohort obesity overfeeding polyunsaturated fat prediction saturated fat
2018/11/13
Background: Dietary fat composition can affect ectopic lipid accumulation and, thereby, insulin resistance. Diets that are high in saturated fatty acids (SFAs) or polyunsaturated fatty acids (PUFAs) h...
Repeats as global DNA methylation marker in bovine preimplantation embryos
epigenetics retrotransposon bisulfite sequencing
2017/2/21
DNA methylation undergoes dynamic changes and is a crucial part of the epigenetic regulation during mammalian early development. To determine the DNA methylation levels in bovine embryos, we applied a...
基于线粒体COI的DNA条形码在对虾科种类鉴定中的研究
DNA条形码 COI基因 对虾鉴别
2019/1/21
对虾科(Penaeidae)包含有26个属，约有200多种对虾，由于同属内的对虾在形态上非常相似，只呈现细微的差别，因此使得只基于形态学对对虾科物种的鉴别非常困难。为确定DNA条形码技术在对虾科物种鉴别的可行性，在本研究中，我们采用线粒体细胞色素c氧化酶亚基I（COI）基因研究了32种对虾的核苷酸组成、对虾种间及种内遗传距离，用邻接法( Neighbor-joining) 构建32种对虾属 COI...
近年来DNA条形码的广泛应用推动了包括分类学在内的多学科的发展，促进了进化生物学及生态学的研究，受到高度关注。DNA条形码不仅能够简单地用于物种识别，还可以用于动物食性、食物链及食物网的研究，主要通过提取粪便及肠道内容物的短片段DNA来得到实现。本文阐述了DNA条形码的研究概况及其在植食性昆虫食性鉴定中的应用，并详细介绍了用于植食性昆虫食性鉴定的DNA条形码的选择，最后针对鳞翅目幼虫食性相关研究予...
应用SELEX技术检测水稻RFL转录因子的DNA结合位点
水稻 转录因子 SELEX DNA结合位点
2018/6/28
FLORICAULA/LEAFY是植物特有的转录因子, 其可以通过调控下游基因的表达对植物的发育起到调控作用。水稻(Oryza sativa)中的同源基因RFL (Rice FLORICAULA/LEAFY)能够调控多个发育过程, 包括开花、叶间期以及枝梗和颖花分化。指数富集配体的系统进化(SELEX)技术已经被广泛应用于体外筛选转录因子的DNA结合位点。应用SELEX技术检测水稻RFL的DNA结...
探讨血清S-腺苷甲硫氨酸(SAM)/S-腺苷同型半胱氨酸(SAH)比值在同型半胱氨酸(Hcy)致载脂蛋白E基因敲除(ApoE―/―)小鼠血管动脉粥样硬化(As) B1重复序列DNA甲基化改变中的作用。方法 36只饲养4周龄ApoE―/―小鼠,随机分为三组:模型对照组、高蛋氨酸组、叶酸＋VitB12干预组,每组12只,分别给予不同饮食；另取健康4周龄雄鼠(SPF级C57BL/6J) 12只作为正...
DNA-Based Patterning of Tethered Membrane Patches
DNA Lipids Lipid Bilayers Molecular Structure Particle Size Surface Properties
2016/5/25
Solid-supported lipid bilayers are useful model systems for mimicking cellular membranes; however, the interaction of the bilayer with the surface can disrupt the function of integral membrane protein...
Individual Vesicle Fusion Events Mediated by Lipid-anchored DNA
Cholesterol DNA Lipid Bilayers Phosphatidylcholines Phosphatidylethanolamines Membrane Fusion Unilamellar Liposomes
2016/5/25
Membrane fusion consists of a complex rearrangement of lipids and proteins that results in the merger of two lipid bilayers. We have developed a model system that employs synthetic DNA-lipid conjugate...
Formation and Analysis of Topographical Domains Between Lipid Membranes Tethered by DNA Hybrids of Different Lengths
Cell Membrane DNA Membrane Lipids Lipid Bilayers 1,2-Dipalmitoylphosphatidylcholine Microscopy, Fluorescence Base Sequence Molecular Sequence Data Models,Chemical Unilamellar Liposomes
2016/5/25
We recently described a strategy to prepare DNA-tethered lipid membranes either to fixed DNA on a surface or to DNA displayed on a supported bilayer [Boxer et al., J. Struct. Biol., 2009, 168, 190; Bo...
Stability of DNA-Tethered Lipid Membranes with Mobile Tethers
Metallic glasses Rapid-solidification Microstructure Mechanical properties
2016/5/25
We recently introduced two approaches for tethering planar lipid bilayers as membrane patches to either a supported lipid bilayer or DNA-functionalized surface using DNA hybridization (Chung, M.; Lowe...
Covalent Attachment of Lipid Vesicles to a Fluid-Supported Bilayer Allows Observation of DNA-Mediated Vesicle Interactions
Caspian and Black Sea waters reverse-osmotic desalination Na-zeolite softening of the permeate
2016/5/25
Specific membrane interactions such as lipid vesicle docking and fusion can be mediated by synthetic DNA-lipid conjugates as a model for the protein-driven processes that are ubiquitous in biological ...
DNA-tethered Membranes Formed by Giant Vesicle Rupture
Tethered lipid membrane Surface immobilized DNA Giant unilamellar vesicles
2016/5/25
We have developed a strategy for preparing tethered lipid bilayer membrane patches on solid surfaces by DNA hybridization. In this way, the tethered membrane patch is held at a controllable distance f...
Effects of Linker Sequences on Vesicle Fusion Mediated by Lipid-Anchored DNA Oligonucleotides
DNA machine synthetic biology
2016/5/25
Synthetic lipid-oligonucleotide conjugates inserted into lipid vesicles mediate fusion when one population of vesicles displays the 5'-coupled conjugate and the other the 3'-coupled conjugate, so that...
Lipid-anchored DNA Mediates Vesicle Fusion as Observed by Lipid and Content Mixing
DNA Vesicles Lipids Fuses
2016/5/25
A general method for synthesizing 5(')- and 3(')-coupled DNA-lipid conjugates has been developed and employed in DNA-mediated vesicle fusion. Vesicles presenting complementary DNA fuse, resulting in b...
Kinetics of DNA-mediated Docking Reactions Between Vesicles Tethered To Supported Lipid Bilayers
DNA electron transfer oxidative stress redox regulation
2016/5/25
Membrane-membrane recognition and binding are crucial in many biological processes. We report an approach to studying the dynamics of such reactions by using DNA-tethered vesicles as a general scaffol...