搜索结果: 76-90 共查到“知识库 生物学 DNA”相关记录887条 . 查询时间(0.613 秒)
为评价分子佐剂细胞毒性T淋巴细胞相关抗原4(CTLA-4)对IBDV VP2质粒DNA的免疫增强作用，试验采用重组质粒载体表达重组蛋白mLTA-VP2-CTLA-4和mLTA-CTLA-4，家兔毒性试验确定其安全性，后选择10日龄非免疫健康鸡进行随机分组试验，设不同剂量mLTA-CTLA-4加IBD活疫苗免疫组、不同剂量mLTA-VP2-CTLA-4免疫组、IBD活疫苗免疫组及空白对照组，免疫后用...
Design, Synthesis and Study of DNA-Targeted Benzimidazole-Amino Acid Conjugates
DNA minor groove Benzimidazole Amidine
2015/5/21
The DNA minor groove continues to be an important biological target in the development of anticancer, antiviral, and antimicrobial compounds. Among agents that target the minor groove, studies of well...
Analysis of the Spatiotemporal Localization of Mitochondrial DNA Polymerases of Trypanosoma brucei
cell cycle DNA polymerase kinetoplast DNA microscopy mitochondrion Trypanosomes
2015/1/28
The mitochondrion contains its own genome. Replication of the mitochondrial DNA (mtDNA) is an essential process that, in most organisms, occurs through the cell cycle with no known mechanism to ensure...
Field surveys utilizing indirect indicators are often adopted in population ecology studies of wild cats in China．However,research using molecular biology techniques are still relatively rare． Confirm...
Regulocator: A Novel Method for the Identification of Conserved Elements in Genomic DNA
ReguLocator DNA Conserved Elements
2015/1/9
The process of generating RNA message from a gene is termed "transcription," and is performed by aptly named "transcription factors." These transcription factors can function singly or in conjunction ...
牛Ascl2 基因印记及DNA甲基化状态分析
印记状态 DNA甲基化 差异甲基化
2014/12/29
为探讨Ascl2基因在成年牛不同组织中的印记状态，并揭示DNA甲基化在调控Ascl2基因印记中的可能作用。本研究首先应用基于核苷酸多态的RT-PCR产物直接测序法对Ascl2基因在牛7个组织（心、肝、脾、肺、肾、肌肉和脂肪）中的表达及印记状态进行了分析，进一步应用亚硫酸氢盐测序法分析牛肺、肝和肾组织中Ascl2基因启动子区的等位基因特异的甲基化状态。RT-PCR产物测序结果显示，Ascl2基因印记...
The HFD of Cse4p is sufficient for a functional centromere specific nucleosome: An investigation into kinetochore formation on cen DNA
Centromere chromosomes cell division protein chromosome axis of fiber
2014/12/11
Centromeres are the site on a chromosome that allows for proper chromosome segregation during cell division. The kinetochore, a DNA-protein complex that forms at the centromere, attaches the chromosom...
The Role Of ERα, ERβ And Phytoestrogens From Soy In P53- Mediated Response To Dna Damage In Mammary Epithelium
Biological sciences Breast cancer Estrogen receptors Mammary tumors p53 Phytoestrogens DNA damage
2014/12/11
Estrogenic compounds can stimulate proliferation of the mammary epithelium, but also potentiate the activity of the p53 tumor suppressor protein. These contradictory activities of estrogenic compounds...
Mitochondrial DNA Polymerase IB: Functional Characterization of a Putative Drug Target for African Sleeping Sickness
kDNA kinetoplast DNA mitochondria RNA interference trypanosoma brucei trypanosome
2014/12/10
Trypanosoma brucei and related parasites are causative agents of severe diseases that affect global health and economy. T. brucei is responsible for sleeping sickness in humans (African trypanosomiasi...
芡实多糖的提取、抗氧化活性及对质粒DNA氧化损伤防护作用的研究
芡实 提取 抗氧化活性 DNA氧化损伤
2016/7/1
采用纤维素酶超声辅助法提取芡实多糖,由正交实验获得芡实多糖的最佳提取条件,并探究其体外抗氧化活性及对H2O2光解反应诱导质粒p BR322 DNA损伤的保护作用。结果表明芡实多糖的最佳提取条件为:料液比1∶20(g/m L)、p H3、纤维素酶用量为原料质量的2.0%、超声时间5min、提取时间3h、提取温度40℃,多糖得率为17.04%。芡实多糖清除DPPH·、羟基自由基的能力较好,IC50分别...
Evaluation of the IrisPlex DNA-based eye color prediction tool in the United States
DNA forensic biology genotype data single nucleotide polymorphisms unknown biological samples the scene of the crime
2015/5/22
DNA phenotyping is a rapidly developing area of research in forensic biology. Externally visible characteristics (EVCs) can be determined based on genotype data, specifically from single nucleotide po...
研究ESAT6、CFP10基因DNA疫苗分别与卡介苗联合免疫小鼠, 以诱导免疫效果。方法 以构建的真核表达载体pEGFP-N1-ESAT6、pEGFP-N1-CFP10与卡介苗共同免疫随机分成8组的80只小鼠, 分别标记为:生理盐水组(SLYS)、卡介苗组(KJM)、pEGFP-N1组(P-N1)、pEGFP-N1-ESAT6组(P-N1-E)、pEGFP-N1-CFP10组(P- N1-C)、卡...
Association of DNA Methylation Levels with Tissue-specific Expression of Adipogenic and Lipogenic Genes in Longissimus dorsi Muscle of Korean Cattle
Adipogenesis DNA Methylation Intramuscular Fat Korean Cattle
2016/5/17
Epigenetic factors, such as DNA methylation status, may regulate adipogenesis and lipogenesis, thus affecting intramuscular fat (IMF) deposition in longissimus dorsi muscle (LM) of beef cattle. In Kor...
传统发酵牦牛酸乳宏基因组DNA三种提取方法比较研究
微生物 传统发酵牦牛酸乳 宏基因组 DNA
2016/12/22
微生物区系复杂,自然环境中约95%是无法用常规的培养方法获得的,为了对未能培养或不可培养的微生物进行研究,本实验选用传统发酵牦牛酸乳,分别采用CTAB-SDS-冻融法、液氮研磨法、玻璃珠吸附法来提取宏基因组DNA,然后测得三种方法所提取的DNA的浓度、纯度和片段分布状况,并对总DNA进行16S r RNA基因片段PCR扩增。实验结果表明,本实验所采取的三种DNA提取方法均能提取到适于后续研究质量的...