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为了解辽宁法库叶茂台出土辽代丝绸的老化程度,应用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法(简称SDS-PAGE)分析辽代丝绸蛋白质的相对分子量,并依据与现代蚕丝蛋白质组成分子量的对比结果来探讨辽代丝绸的老化特征。SDS-PAGE结果表明辽代丝绸蚕丝蛋白质残留着相对分子量为65kD和56kD两条肽链,而蚕丝蛋白质通常含有的25kD轻链蛋白却并不存在,其丝绸组成已经发生了明显变化,说明辽代丝绸糟朽严重,并经...
利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)分离了银杏种子中的糖蛋白组分,进一步用氨水催化释放N-糖链,并采用电喷雾离子质谱(ESI-MS)及在线液相色谱-质谱联用(LC-UV-MS/MS2)等技术对胶条上释放的N-糖链进行了定性定量分析.结果表明,从银杏种子中分离得到11种糖蛋白,共检测到11条N-糖链,包括高甘露糖型(4.88%)和复杂型(95.12%)2种类型,其中分子量为210...
Various methods of analysis and dataset have been used for urban studies. However, used urban data often has low spatial resolution,especially regional statistics, though statistical data can cover la...
When Robert Broderick Le Page passed away in 2008, he le! behind a rich legacy of contributions – descriptive, theoretical and applied – to sociolinguistics and creole studies.1 His work is especially...
This study was carried out to determine the whole cell protein profiles of Salmonella serovars from chicken, turkey and sheep faeces by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-P...
Abstract: We consider the problem of the convergence of the so-called Le Page series in the Skohorod space $\bbD^d=\bbD([0,1],\bbR^d)$ and provide a simple criterion based on the moments of the increm...
The central father and daughter figures of P.K. Page’s "War Lord in the Early Evening" stand together in a formal garden, a poetic space that will be familiar to readers of Page’s work. Both Suniti Na...
The historical conflict between North American Natives and European colonists roughly parallels the debate between orality and literacy that has been waged since writing first developed and irrevocabl...
Although many factors determine Google's overall ranking of search engine results, Google maintains that the heart of its search engine software is PageRank, the eigenvector corresponds to the maximal...
Many commercial and research WWW browsers include a variety of graphical revisitation tools that let users return to previously seen pages. Examples include history lists, bookmarks and site maps. In ...
依据国际种子检验协会(ISTA)1986年公布的标准小麦醇溶蛋白酸性聚丙烯酰胺凝胶电泳(A-PAGE)方法,结合国内实验室的实际情况,摸索出了适合我国试剂和仪器的小麦醇溶蛋白酸性聚丙烯酰胺凝胶电泳(A-PAGE)的改良方法。该方法克服了标准方法中的胶韧性差、剥胶困难、分辨率不高、谱带识别繁琐、实验效率较低的限制,适合我国小麦品种纯度的快速鉴定、血缘关系分析等。
研究谷子贮藏蛋白多态性对谷子品种选育和鉴定评价具有重要的意义。本文利用A-PAGE方法对来自不同生态区的24份谷子品种籽粒的水溶蛋白、盐溶蛋白、酸溶蛋白和醇溶蛋白的多态性进行了研究。结果发现,不同谷子品种之间籽粒水溶蛋白、酸溶蛋白谱带相似,基本没有差异;每个谷子品种的盐溶蛋白谱带有20多条,仅有4个品种出现多态性谱带,多态性也较低。说明谷子品种水溶蛋白、盐溶蛋白和酸溶蛋白A-PAGE谱带不适用于谷...
The purpose of this article is to draw your attention to the information that is available in the field of human resources development, and to explain how to find it. We regard it as a valuable resour...

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