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Effects of chilled storage and pH of activating solution on different motility parameters in burbot (Lota lota) sperm
short-term storage activation Lota lota sperm analysis CASA parameters
2018/11/23
The effects of a simple saline solution prepared using two different pH (4.4 and 8.5) on sperm motility in burbot were investigated. Results were recorded during a 96-hour chilled storage (4°C) in 24-...
Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes
bovine semen large volumes semen processing sperm quality parameters
2018/11/23
The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact me...
The Usefulness of Selected Physicochemical Indices, Cell Membrane Integrity and Sperm Chromatin Structure in Assessments of Boar Semen Sensitivity
Boar Chromatin Structure Sperm Membrane Integrity Sperm Morphology
2016/5/19
The present work describes experiments undertaken to evaluate the usefulness of selected physicochemical indices of semen, cell membrane integrity and sperm chromatin structure for the assessment of b...
Characterization of the sperm factor responsible for initiating [Ca2+]i oscillations during fertilization in mammalian eggs
Biological sciences Sperm factor Oscillations Fertilization Mammalian eggs
2015/1/28
During fertilization the sperm activates the mammalian egg by eliciting [Ca2+]i oscillations. However, how the sperm triggers [Ca2+]i oscillations in mammalian eggs remains unknown. To test the possib...
Characterization and phosphorylation of an MPM-2 reactive sperm protein complex involved in zygotic aster formation
Sperm tubes egg quality microtubules female pronucleus male pronucleus fertilized egg triplet protein
2014/12/11
The zygotic centrosome, contributed by the sperm, forms an array of microtubules that spread throughout the ooplasm. These microtubules are used by the female pronucleus to come into juxtaposition wit...
Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction
Sex Determination Sexed Semen Multiplex Real-time polymerase chain reaction
2016/5/17
Gender selection is important in livestock industries; for example, female calves are required in the dairy industry. Sex-sorted semen is commonly used for the production of calves of the desired gend...
L-carnitine Supplemented Extender Improves Cryopreserved-thawed Cat Epididymal Sperm Motility
L-carnitine Epididymal Spermatozoa Cryopreservation Cat
2016/5/16
Cryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detr...
Production of Transgenic Goats by Sperm-mediated Exogenous DNA Transfer Method
Sperm-mediated Gene Transfer Goat Sperm Cryopreservation Artificial Insemination (AI)
2016/5/3
In this study, the production of transgenic goats using sperm to integrate exogenous DNA and artificial insemination (AI) was carried out and the technical protocols for sperm-mediated gene transfer (...
Near-future levels of ocean acidification do not affect sperm motility and fertilization kinetics in the oyster Crassostrea gigas
Near-future levels ocean acidification sperm motility and fertilization kinetics the oyster Crassostrea gigas
2010/1/21
An increasing number of studies are now reporting the effects of ocean acidification on a broad range of marine species, processes and systems. Many of these are investigating the sensitive early life...
Intraspecific divergence in sperm morphology of the green sea urchin, Strongylocentrotus droebachiensis: implications for selection in broadcast spawners
sperm morpholog sea urchin,
2015/12/11
Sperm morphology can be highly variable among species, but less is known about
patterns of population differentiation within species. Most studies of sperm morphometric
variation are done in species...
Comparison of Viability, ATP and In vitro Fertilization of Boar Sperm Stored at 4??C in the Three Different Diluents
Fertilization Liquid Semen Oocyte Porcine
2016/4/28
This study was designed to develop a method of liquid storage of boar sperm at 4??C by using the modified Beltsville F5 (BF5) diluent with bovine serum albumin (BSA) and N-acetyl-D-glucosamine. Boar s...
Comparison of Genetic Parameter Estimates of Total Sperm Cells of Boars between Random Regression and Multiple Trait Animal Models
Genetic Correlation Boar Semen Random Regression Model Multiple Trait Animal Model
2016/4/28
The objective of this study was to compare random regression model and multiple trait animal model estimates of the (co) variance of total sperm cells over the active lifetime of AI boars. Data were p...
Effects of Sperm Membrane Disruption and Electrical Activation of Oocytes on In vitro Development and Transgenesis of Porcine Embryos Produced by Intracytoplasmic Sperm Injection
Intracytoplasmic Sperm Injection GFP Transgenic Pigs
2016/4/27
The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species ...
Maintenance of Sperm Characteristics and In vitro Developmental Rate of Embryos against Oxidative Stress through Antioxidants in Pig
Reactive Oxygen Species (ROS) Boar Semen Taurine Embryo Antioxidant Sperm Characteristic
2016/4/27
Oxidative stress is one of the major causes of failure of in vitro storage of boar semen. Reactive oxygen species (ROS) are one of the important mediators of oxidative stress during in vitro storage o...
Capacitation-associated Changes in Protein-tyrosine-phosphorylation, Hyperactivation and Acrosome Reaction in Guinea Pig Sperm
Acrosome Reaction Capacitation Hyperactivation Tyrosine Phosphorylation Semen
2016/4/27
The aim of this study was to evaluate the effects of Ca2+, HCO3- and BSA on the in vitro capacitation-associated protein tyrosine phosphorylation, hyperactivation and acrosome reaction in guinea pig s...