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浙江大学生命科学研究院2021年3月16日范衡宇课题组在Advanced Science杂志发表论文阐明CCR4-NOT复合体中RNA识别亚基CNOT4在哺乳动物生殖细胞性染色体配对中的生理功能（图）
范衡宇 CCR4-NOT复合体 RNA识别 动物生殖细胞 性染色体配对 生理功能
2022/10/26
2021年3月16日，浙江大学生命科学研究院范衡宇课题组在Advanced Science杂志上在线发表了题为“The CNOT4 Subunit of the CCR4-NOT Complex is Involved in mRNA Degradation, Efficient DNA Damage Repair, and XY Chromosome Crossover during Male ...
长链非编码RNA(Long noncoding RNAs，lncRNA)是一类转录本长度超过200 nt的非编码RNA分子，它们以RNA的形式在表观遗传调控、转录调控以及转录后调控等多层面调控基因的表达。它们参与细胞增殖、分化和凋亡等多种生物学过程。近年来，lncRNA在畜禽动物中的研究越来越受到人们的重视，人们发现lncRNA在动物生长发育中扮演着很重要的角色。本文对近十五年来畜禽动物如猪、鸡、...
Characterizing Milk Production Related Genes in Holstein Using RNA-seq
RNA-seq Holstein Milk Production Meta-analysis Milk Yield Differentially Expressed Gene
2016/5/19
Although the chemical, physical, and nutritional properties of bovine milk have been extensively studied, only a few studies have attempted to characterize milk-synthesizing genes using RNA-seq data. ...
Monitoring of Chicken RNA Integrity as a Function of Prolonged Postmortem Duration
Gallus gallus Skeletal Muscle Postmortem Duration RNA Integrity Gene Expression
2016/5/19
Gene expression profiling has offered new insights into postmortem molecular changes associated with meat quality. To acquire reliable transcript quantification, high quality RNA is required. The obje...
RNA-seq Profiles of Immune Related Genes in the Spleen of Necrotic Enteritis-afflicted Chicken Lines
RNA-seq Chicken Necrotic Enteritis Cytokine Eimeria maxima Clostridium perfringens
2016/5/19
The study aimed to compare the necrotic enteritis (NE)-induced transcriptome differences between the spleens of Marek’s disease resistant chicken line 6.3 and susceptible line 7.2 co-infected with Eim...
Phosphorylation Status of RNA Polymerase II Carboxyl-terminal Domain in Porcine Oocytes and Early Embryos
RNA Polymerase II Porcine Oocyte Embryos α-Amanitin
2016/5/10
Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryoni...
Validation of Gene Silencing Using RNA Interference in Buffalo Granulosa Cells
RNAi Transfection CYP19 Gene 17beta- Estradiol Buffalo Transcript Abundance
2016/5/9
Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is re...
Targeted Suppression of Connexin 43 in Ovine Preimplantation Embryos by RNA Interference Using Long Double-stranded RNA
Connexin 43 RNA Interference Ovine Embryo
2016/5/4
RNA interference (RNAi) is an acknowledged useful and effective tool to study gene function in various cells. Here, we suppressed the Connexin 43 (Cx 43) gene expression during in vitro development of...
小鼠胚胎诱导型Hsp70基因的RNA干扰有效序列筛选
RNA干扰 小鼠胚胎成纤维细胞 小干扰RNA
2009/12/3
【目的】应用RNA干扰技术沉默小鼠胚胎成纤维细胞（Mouse embryonic fibroblast，MEF）中诱导型热休克蛋白70（Hsp72）基因的表达，从而筛选出对Hsp72基因具有确实干扰作用的siRNA。【方法】设计并合成4对特异性针对Hsp72基因的siRNA，借助LipofectamineTM 2000瞬时转染MEF，41℃热应激处理后，采用RT-PCR和Western blot检...
RNA干扰（RNAi）是指小分子双链RNA通过特异性降解与其同源的mRNA，而在mRNA水平上高效阻断体内特异性基因表达的现象，属于转录后水平基因沉默。利用该技术进行基因功能研究，经济有效，通用性好，已成为反向遗传学研究中最重要的工具之一。在动物繁殖领域，RNAi技术主要应用于体外研究哺乳类和禽类卵母细胞发育、胚胎发育和精子形成中重要基因的功能及其作用机制。随着该技术不断发展完善，RNAi必将在动...
RNA干涉（RNAi）技术应用于哺乳动物细胞的研究策略
RNA干扰 siRNA 基因功能分析 哺乳动物细胞
2008/2/1
摘要RNAi作为新近发展起来的基因功能分析技术，近年来在哺乳动物细胞中的研究已取得了长足进展，且有着广泛的应用前景。对RNAi作用机制及RNAi实验操作技术的探讨是目前研究的热点。研究表明，哺乳动物细胞中的RNAi作用模式与植物有所不同。文章对RNAi作用机制、哺乳动物细胞RNAi实验的一般策略（包括靶siRNA序列选择、siRNA获取方法、siRNA转染、RNAi效果检测等）以及最新研究进展进行...
双壳纲动物核糖体RNA 18S-ITS1序列及其在分子系统发育研究中的应用18S-ITS1 Sequence of rRNA in Bivalves and its Application in Phylogenetic Analysis
ITS1 18S RNA 遗传多样性 双壳纲
2008/1/20
摘要
测定了双壳纲不同科、属、种及种内共11个个体的核糖体RNA 18S-ITS1序列。结果表明，该序列在种间存在很高的多态性，长度从558 bp到784 bp不等，碱基差异百分比在10.7%~61.7%之间， ITS1序列同源性很低，有片段的插入与缺失。种间18S部分序列碱基差异百分比在0.9%~23.7%之间，变化主要是碱基的转换。用邻接法（NJ）构建了8个种的18S部分序列（约240 bp...
题目：茶尺蠖小RNA病毒5'端非编码区的克隆和测序及与哺乳动物小RNA病毒的比较分析
作者：王小纯1,2， 张珈敏1， 蒋洪1， 俞海洋1， 谭莉1， 胡远扬1*
摘要：用Trizol从纯化的茶尺蠖Ectropis oblique小RNA病毒（EoPV）中提取病毒基因组RNA，逆转录后加poly(dT)，然后进行两步PCR扩增基因组5′端。克隆测序后，对其5′端非编码区的核苷酸序列进行分析，发现...
Application of Recent DNA/RNA-based Techniques in Rumen Ecology
Rumen Microbial Ecology, Ribosomal Molecular Functional Analysis
2016/4/25
Conventional culture-based methods of enumerating rumen microorganisms (bacteria, archaea, protozoa, and fungi) are being rapidly replaced by nucleic acid-based techniques which can be used to charact...