搜索结果: 1-8 共查到“知识库 生物学其他学科 DNA”相关记录8条 . 查询时间(0.734 秒)
Regulocator: A Novel Method for the Identification of Conserved Elements in Genomic DNA
ReguLocator DNA Conserved Elements
2015/1/9
The process of generating RNA message from a gene is termed "transcription," and is performed by aptly named "transcription factors." These transcription factors can function singly or in conjunction ...
DNA改组在腺相关病毒定向进化中的发展及其应用
DNA改组 AAV 衣壳蛋白
2013/8/9
DNA改组(DNA shuffling)是一种高通量的突变、筛选技术, 自1994年提出以来, 经过了几十年的发展, 其在DNA、酶和药物蛋白等的改造上都有突出的表现. 腺相关病毒(adeno- associated virus, AAV)是一种细小病毒, 由于其无致病性和能有效呈递基因而成为一种非常理想的基因表达载体. 但是AAV筛除存在一些不足, 如对某些重要靶细胞感染效率不高、存在机体的免疫...
DNA Sequencing via Quantum Mechanics and Machine Learning
DNA Sequencing Quantum Mechanics Machine Learning
2011/1/5
Rapid sequencing of individual human genome is prerequisite to genomic medicine, where diseases will be prevented by preemptive cures. Quantum-mechanical tunneling through single-stranded DNA in a sol...
Towards a theoretical understanding of false positives in DNA motif finding
theoretical understanding false positives DNA motif finding
2011/1/5
Detection of false-positive motifs is one of the main causes of low performance in motif finding methods. It is generally assumed that false-positives are mostly due to algorithmic weakness of motif-f...
Accurate prediction of gene expression by integration of DNA sequence statistics with detailed modeling of transcription regulation
gene expression DNA sequence statistics detailed modeling transcription regulation
2011/1/5
Gene regulation involves a hierarchy of events that extend from specific protein-DNA interactions to the combinatorial assembly of nucleoprotein complexes. The effects of DNA sequence on these process...
绵羊体细胞克隆胚胎的异常DNA甲基化模式
甲基化 抗5-甲基胞嘧啶抗体 绵羊 体外受精 体细胞克隆
2013/9/17
以体外受精胚胎作为对照, 利用抗5-甲基胞嘧啶抗体免疫荧光法对绵羊体细胞克隆胚胎的基因组甲基化模式进行了分析. 实验结果表明: (ⅰ) 在着床前发育过程中, 体细胞克隆胚胎呈现出与体外受精胚胎类似的去甲基化趋势, 即在8-细胞期甲基化水平降到最低点, 紧接着在桑椹胚时又升高, 但是克隆胚胎的甲基化水平明显高于同一时期的体外受精胚胎, 特别在8-细胞期及其以后时期; (ⅱ) 克隆囊胚的甲基化模式与体...
红山茶高质量基因组DNA的提取
基因组DNA提取 ITS序列测定 红山茶
2012/10/10
红山茶植物细胞含有大量的多糖、脂质、色素和酚类物质，严重影响基因组DNA的提取.本研究在多次实验的基础上对CTAB法进行了改良，结果表明：采用细胞核被解裂之前加入不溶性聚乙烯吡咯烷酮(PVP)，用冰冻异丙醇沉淀基因组DNA等关键技术，可以从山茶属红山茶组56个物种的植物叶片中快速提取高质量的DNA，满足ITS序列测定的需要；用嫩叶提取可获得纯度较高的DNA；样品在-20℃低温下保存对DNA的提取质...