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Effect of ZnO nanoparticle on cell viability,zinc uptake efficiency,and zinc transporters gene expression:a comparison with ZnO and ZnSO4
nano-ZnO bulk ZnO zinc transportation TPEN IEC-6 cells
2017/2/21
Zinc plays an important role in functional and structural integrity of cells. The aim of the current study was to compare cell viability, zinc uptake efficiency, and gene expression of metallothionein...
为了探究不同填料生物膜上微型动物物种多样性异同，研究采用3种填料（多面空心球、K3悬浮填料、煤焦粉）处理模拟生活污水时微型动物多样性指数变化规律及其与水处理效果的相关性。结果表明：生物膜中共出现微型动物57种，包括鞭毛虫7种、肉足虫5种、纤毛虫42种和微型后生动物3种；煤焦粉上以小型鞭毛虫为优势类群，多面空心球与K3悬浮填料上由小型鞭毛虫、游泳型纤毛虫和固着型纤毛虫交替构成优势类群；悬浮型填料、堆...
Transcriptome profile of one-month-old lambs’ granulosa cells after superstimulation
Lamb Granulosa Cells Sheep RNA Seq Superstimulation
2017/3/7
Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte m...
Random Regression Models Using Legendre Polynomials to Estimate Genetic Parameters for Test-day Milk Protein Yields in Iranian Holstein Dairy Cattle
Holstein Dairy Cattle Milk Protein Yields Random Regression Model Test-day Records
2016/12/28
The objective of this study was to estimate the genetic parameters of milk protein yields in Iranian Holstein dairy cattle. A total of 1,112,082 test-day milk protein yield records of 167,269 first la...
Association of the Single Nucleotide Polymorphisms in RUNX1, DYRK1A, and KCNJ15 with Blood Related Traits in Pigs
Positional Candidate Gene Blood Related Traits Association Test Landrace Korean Native Pigs
2016/12/28
The aim of this study was to detect positional candidate genes located within the support interval (SI) regions based on the results of red blood cell, mean corpuscular volume (MCV), and mean corpuscu...
基于线粒体COI的DNA条形码在对虾科种类鉴定中的研究
DNA条形码 COI基因 对虾鉴别
2019/1/21
对虾科(Penaeidae)包含有26个属，约有200多种对虾，由于同属内的对虾在形态上非常相似，只呈现细微的差别，因此使得只基于形态学对对虾科物种的鉴别非常困难。为确定DNA条形码技术在对虾科物种鉴别的可行性，在本研究中，我们采用线粒体细胞色素c氧化酶亚基I（COI）基因研究了32种对虾的核苷酸组成、对虾种间及种内遗传距离，用邻接法( Neighbor-joining) 构建32种对虾属 COI...
Genetic diversity within and gene flow between three draught horse breeds using genealogical information
genetic drift inbreeding Silesian Noriker Noriker Czech-Moravian Belgian horse
2016/11/11
Three draught horse breeds are maintained in the Czech Republic: the Silesian Noriker (SN), the Noriker (N), and the Czech-Moravian Belgian (CMB). Because the SN and CMB populations are currently clos...
Effects of thermal manipulation during late incubation period on post-hatch thermotolerance in ostrich
ostrich embryo heat stress hatchability blood biochemicals epigenetic
2016/11/11
The effects of thermal manipulation (TM) during late ostrich embryonic development on hatchability, body weight, biochemical and hormonal changes, and the ability of hatching chicks to cope with therm...
本试验旨在探究刚地弓形虫钙依赖蛋白激酶9（CDPK9）对小鼠Ana-1巨噬细胞活性及功能的影响。利用激光共聚焦技术验证CDPK9与Ana-1巨噬细胞的结合情况；采用细胞计数试剂盒（cell counting kit-8，CCK-8）检测不同浓度CDPK9处理24 h对Ana-1巨噬细胞增殖活性的影响；利用流式细胞术检测不同浓度CDPK9处理48 h对Ana-1巨噬细胞凋亡及吞噬功能的影响；采用总一...
随着第二代测序技术的研发和应用，基因组学的研究不断出新，为其带来了更新的科研方法和解决方案。基因组测序可以更深地了解一个物种的分子进化、基因组成和基因调控等特点，特别基因组重测序技术的发展和应用，将基因组学的研究推向了多领域、多样化、多功能的新阶段。现已从变异检测、性状定位、遗传图谱构建、群体进化分析等方面取得丰硕成果。文章阐述了动物基因组重测序学领域中全基因组测序技术和简化基因组测序技术的应用现...
奶牛分子系谱构建或亲权鉴定的微卫星标记筛选
中国荷斯坦牛 微卫星位点 排除概率 亲子鉴定
2017/5/3
旨在利用较少的经过优化的遗传标记组合达到快速高效的亲缘鉴定，最终可有效应用于奶牛群体亲子鉴定与系谱构建。本研究从国际动物遗传学会（ISAG）数据库中初步选择了18个多态性较高的微卫星位点，经PCR优化验证获得可高效扩增的8个位点，并检测其在300头中国荷斯坦牛群体中的多态性分布。这些微卫星位点的亲子鉴定效力检测结果表明，8个标记平均等位基因数为14.63，平均多态性信息含量（PIC）为0.747，...
以SBR反应器培养驯化阶段的活性污泥微型动物群落为研究对象，系统考察了活性污泥驯化过程中微型动物群落结构与优势种群变化规律，同时探讨了微型动物群落物种多样性及其稳定性.结果表明，1活性污泥培养驯化是微型动物群落类群和种属增多后趋于稳定的过程：污泥培养驯化过程中微型动物群落，由驯化初期以鞭毛虫为单一优势类群向鞭毛虫、匍匐型纤毛虫、固着型纤毛虫、肉食性纤毛虫等4个共优势类群演变，优势种属也由单一的波豆...
染色质重塑因子BPTF、BRG1在不同毛色绵羊皮肤的表达与定位
BPTF BRG 绵羊 毛色
2017/4/1
旨在研究染色质重塑因子BPTF、BRG1在不同毛色绵羊皮肤的表达与定位。采集黑色和白色各3只绵羊的背部皮肤组织，用qRT-PCR检测不同毛色皮肤中BPTF和BRG1 mRNA相对表达量，免疫组化技术定位分析，Western blotting半定量研究BPTF和BRG1蛋白相对表达量。qRT-PCR结果表明，BPTF基因在黑色绵羊皮肤中的mRNA相对表达量显著高于白色绵羊（P<0.05），BRG1基...
Characterization of the Methylation Status of Pax7 and Myogenic Regulator Factors in Cell Myogenic Differentiation
Pax7 Myogenic Regulatory Factors C2C12 DNA Methylation CpG Islands
2016/7/27
Epigenetic processes in the development of skeletal muscle have been appreciated for over a decade. DNA methylation is a major epigenetic modification important for regulating gene expression and supp...
The Effects of Dietary Phosphorus on the Growth Performance and Phosphorus Excretion of Dairy Heifers
Heifers Growth Performance Phosphorus Phosphorus Excretion
2016/7/27
The objective of this study was to investigate the effects of reducing dietary phosphorus (P) on the frame size, udder traits, blood parameters and nutrient digestibility coefficient in 8- to 10-month...