搜索结果: 1-15 共查到“知识库 生物学 SNP”相关记录43条 . 查询时间(0.071 秒)
国际上首个橡胶草高密度SNP遗传图谱构建
天然橡胶 工业原料 三叶橡胶树 橡胶草
2023/7/4
天然橡胶是世界四大工业原料之一,巴西三叶橡胶树一直是天然橡胶的主要来源,但由于种植面积限制、生产成本增加、遗传背景狭窄和病虫害严重等因素,橡胶生产逐渐难以满足需求。因此,开发生产天然橡胶的替代资源具有重要的战略意义和经济价值。
鸡胰岛素样生长因子2基因(IGF2)外显子区功能性SNP预测与分析
鸡 IGF2基因 SNP功能预测 生物信息学
2021/3/9
旨在采用生物信息学方法筛选鸡IGF2基因中具有潜在生物学功能的非同义单核苷酸多态性(non-synonymous single nucleotide polymorphisms,nsSNPs)位点,为开展标记辅助选择改良鸡重要经济性状提供理论参考。本研究从dbSNP数据库中检索出鸡IGF2基因的12个nsSNPs位点,利用生物信息学软件SIFT、Polyphen-2、PhD-SNP和SNAP预测其...
旨在探究DCT基因启动子区甲基化水平和SNP突变对山羊毛色的影响,为探索DCT基因调控山羊毛色变化的机理提供理论依据。本研究以山羊为试验动物,对DCT基因启动子区进行CpG岛预测,设计引物对预测的2个CpG岛富集区域进行亚硫酸氢盐甲基化测序,使用甲基化水平分析软件BISMA统计甲基化位点,比较唐山奶山羊(白色)和南江黄羊(黑色品系)两种不同毛色山羊群体DCT基因启动子区甲基化水平差异。克隆DCT基...
基于SLAF-seq技术的木薯SNP标记开发
木薯 分子标记 SLAF-seq SNP
2018/11/27
开发大量木薯(Manihot esculenta)特异分子标记可促进木薯种质资源中优良基因的挖掘和利用。本研究以39份木薯种质资源为材料, 利用特异性位点扩增片段测序(specific-locus amplified fragment sequencing, SLAF-seq)技术进行测序, 结果显示: 本次实验双端比对效率为90.83%, 酶切效率为91.18%; 通过测序获得304.30 Mb...
基于SNP标记的桃矮化基因精细定位
桃 矮化基因 SNP 精细定位
2017/11/9
矮化型桃树体矮小、节间短,是盆栽观赏和砧木育种的重要遗传资源。明确矮化性状形成的遗传机制并对桃矮化基因进行精细定位,是建立目标性状分子辅助选种体系和遗传改良的前提,可为有目标的选育矮化观赏桃和砧木品种奠定基础。【方法】以‘05-2-144’(‘97矮’ב鸳鸯垂枝’桃)套袋自交获得的395个后代单株构建的分离群体为材料。参考桃基因组信息并基于Sanger技术开发的SNP标记对亲本和后代单株进行分析...
旨在解析中国地方犬全基因组拷贝数变异(CNV)分布情况,为分析CNV对犬表型变异的影响提供前期基础。本研究采集了16个来自中国不同地方犬品种和9头罗威纳犬共计185头犬的血液样品,使用血液DNA提取试剂盒提取DNA,利用犬170 K SNP芯片和PennCNV软件对中国地方犬进行全基因组范围内的CNV分析。参照Illumina芯片标准操作流程进行芯片扫描和基因分型。将质控后的SNP参考PennCN...
Dynamic expression of HSP90B1 mRNA in the hypothalamus of two Chinese chicken breeds under heat stress and association analysis with a SNP in Huainan chickens
heat shock protein mRNA level single nucleotide polymorphism thermal stress
2017/2/21
The effects of heat stress on HSP90B1 messenger RNA (mRNA) expression in the hypothalamus of chicken were investigated and HSP90B1 variations were detected. Females of two Chinese chicken breeds (Huai...
对于以种子形态为物种主要识别依据的口岸部门,外来入侵杂草长芒苋、西部苋和糙果苋一直是鉴定的难点。本文对34种苋属植物的ITS序列和26S rDNA进行分析,通过SNP变异位点及特异性引物,借助PCR-RFLP方法,对长芒苋、西部苋与糙果苋进行快速、准确的分类鉴定。ITS序列分析表明,长芒苋种内变异小,种间变异显著,可与其他苋属植物明显区分。西部苋与糙果苋之间ITS序列差异小,需依据SNP位点来区别...
本试验旨在研究猪ZP4基因的SNP在群体中的选择压及其与产仔数的关联性。收集大白猪、长白猪、长大二元猪3个群体共计328头母猪的产仔数记录,利用直接测序法克隆ZP4基因中的SNP位点,采用广义显性模型分析ZP4基因SNP与产仔数的关联性。采用lositan软件,Tajima's D检验和Fu and Li's D检验来检测ZP4的选择压。结果发现,ZP4中共检测到27个SNPs,关联分析表明A26...
Association of SNP markers with agronomic and quality traits of field pea in Italy
grain yield marker-assisted selection Pisum sativum protein content QTL
2016/12/8
Only a few studies on pea (Pisum sativum) investigated the association of single nucleotide polymorphisms (SNP) markers with key agronomic traits. This study aimed to explore the association of a stan...
SNP genotyping and population genomics from expressed sequences – current advances and future possibilities
SNP genotyping population genomics
2015/12/11
With the rapid increase in production of genetic data from new sequencing technologies,
a myriad of new ways to study genomic patterns in nonmodel organisms are currently
possible. Because genome as...
SNP Discovery from Transcriptome of Cashmere Goat Skin
Transcriptome Goats Capra hircus RNA Sequencing
2016/5/18
The goat Capra hircus is one of several economically important livestock in China. Advances in molecular genetics have led to the identification of several single nucleotide variation markers associat...
A SNP Harvester Analysis to Better Detect SNPs of CCDC158 Gene That Are Associated with Carcass Quality Traits in Hanwoo
Single Nucleotide Polymorphisms Harvester Method CCDC158 Gene Hanwoo
2016/5/11
The purpose of this study was to investigate interaction effects of genes using a Harvester method. A sample of Korean cattle, Hanwoo (n = 476) was chosen from the National Livestock Research Institut...
A Whole Genome Association Study on Meat Quality Traits Using High Density SNP Chips in a Cross between Korean Native Pig and Landrace
Whole Genome Association SNP Meat Quality Korean Native Pig Landrace
2016/5/10
A whole genome association (WGA) study was performed to detect significant polymorphisms for meat quality traits in an F2 cross population (N = 478) that were generated with Korean native pig sires an...
Identification of the SNP (Single Nucleotide Polymorphism) for Fatty Acid Composition Associated with Beef Flavor-related FABP4 (Fatty Acid Binding Protein 4) in Korean Cattle
Fatty Acid Binding Protein 4 (FABP4) Korean Cattle Unsaturated Fatty Acid Haplotype Beef Flavor
2016/5/10
In this study, we investigated the relationship between unsaturated fatty acids influencing beef flavor and four types of SNPs (c.280A>G, c.388G>A, c.408G>C and c.456A>G) located at exon 2, 3 and 4 of...