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应用创造酶切位点法检测单碱基突变The Establishment of Method for Identifying SNP Genotype by CRS-PCR
CRS-PCR EX-FABP基因 SNP检测
2007/12/30
摘要应用引物错配技术结合单碱基突变位点而配合成一个酶切位点，使之成为可用PCR-RFLP方法分析的突变位点，是对单碱基突变位点进行基因型鉴定的有效而简捷的手段。本文以鸡胞外脂肪酸结合蛋白(Extracelluar fatty acid binding protein,EX-FABP)基因单碱基突变的基因型检测为例，探讨了应用创造酶切位点PCR（Created Restriction Site PC...
四引物PCR扩增反应的单管SNP快速测定法
单核苷酸多型性 聚合酶链反应 等位基因特异性延伸反应 人为错配碱基
2007/12/24
摘要 建立一种在单管中进行单核苷酸多型性 (SNP)快速测定的高效廉价方法 .以人ABCA1基因中的I82 3M为研究对象 ,设计 4种引物进行PCR扩增 ,其中两种引物用于扩增一段含有SNP位点的DNA片段 ,另两种引物为SNP位点特异性引物 ,4种引物在单管中同时进行PCR扩增反应 ,根据延伸产物的长度确定SNP的类型 .为提高SNP测定的特异性 ,在特异性引物的 3′端倒数第 3个碱基引入了...
基于荧光定量PCR扩增反应的SNP测定法
单核苷酸多态性 实时聚合酶链反应 等位基因特异性延伸反应 人为错配碱基
2007/12/21
摘要 建立一种利用荧光定量PCR扩增反应进行单核苷酸多态性(SNP)快速测定的方法.以人β肾上腺素受体2基因中的Arg16Gly为研究对象,利用荧光染料SYBRGreenⅠ标记定量PCR产物,通过PCR生长曲线和融解曲线分析结果进行SNP分型.为提高SNP测定的特异性,分别在野生型和突变型等位基因的特异性引物3′端倒数第3个碱基位置,引入了一个人为错配碱基,使引物的错误延伸率显著降低,大大提高了S...
汉族人群NOS3 A-922G、NOS3 T-786C 与NOS3 G894T SNP的等位基因及其组合分布与高血压的相关性
內皮源性一氧化氮合酶 组合分布 单核苷酸多态性 高血压病 等位基因
2007/12/20
摘要0.05)。男、女性别分层研究：无论男亚组还是女亚组均未发现NOS3 A-922G、NOS3 T-786C 与NOS3 G894T各个位点SNP与高血压病有相关性。等位基因组合分布研究发现NOS3 G894G +A-922G+T-786T组合基因型总体频率分布在高血压病组与正常对照组之间有显著性差异(P0.05)；女亚组中携带NOS3 G894G+A-922G+T-786C 的组合基因型分布频...
DNA聚合酶高保真机理的新发现及其在SNP分析中的应用
DNA聚合酶 校正 分子开关 单碱基多态性
2007/12/5
摘要高保真DNA聚合酶在遗传与进化等生命活动中具有十分重要的生理与病理意义。高保真聚合酶除具有广为人知的校正功能外,最近的实验进一步表明, 由不能及时校正或难于纠正的错配碱基引发的“关”闭DNA聚合反应的效应, 同样保证了DNA聚合反应终产物的纯度。高保真聚合酶这一“关”闭DNA聚合反应的能力, 促成了其与耐外切酶消化的3´末端碱基特异性引物共同构成一个SNP敏感性纳米级复合分子“开/...
广东地区汉族人MBL基因启动子区SNP的研究
2007/7/25
甘露聚糖结合凝集素(mannan-binding lectin, MBL)系C型凝集素超家族中胶凝素家族的重要成员，可识别多种病原体的糖结构，通过调理吞噬和激活补体凝集素途径来清除病原微生物和受感染细胞[1][2]，是机体天然免疫系统中的关键分子[3]。已证实血清MBL水平低下与许多感染性疾病尤其是婴幼儿反复感染、自身免疫病(如系统性红斑狼疮和类风湿性关节炎)以及习惯性流产等有关。MBL由肝细胞合...
Identification of a Novel SNP Associated with Meat Quality in C/EBP Gene of Korean Cattle
C/EBP??Gene SNP Marker Meat Quality Korean Cattle
2016/4/25
CCAAT/enhancer binding protein ??(C/EBP?? plays an important role in lipid deposition and adipocyte differentiation. In order to find genetic markers to improve the meat quality of Korean cattle, the ...
SNP Detection of Carboxypeptidase E Gene and Its Association with Meat Quality and Carcass Traits in Korean Cattle
Carboxypeptidase E Gene SNP Marker Carcass Traits and Meat Quality Korean Cattle
2016/4/25
Carboxypeptidase E (CPE) plays an important role in the regulation of the body fat content. Therefore, it has been suggested as candidate gene for traits related to meat quality in beef cattle. This s...
Association of SNP Marker in the Thyroglobulin Gene with Carcass and Meat Quality Traits in Korean Cattle
TG Gene SNP Marker Marbling Score Korean Cattle
2016/4/25
Thyroid hormones play an important role in regulating metabolism and can affect homeostasis of fat depots. The gene encoding thyroglobulin (TG), producing the precursor for thyroid hormones, has been ...
Association of SNP Marker in the Leptin Gene with Carcass and Meat Quality Traits in Korean Cattle
Leptin Gene SNP Marker Carcass and Meat Quality Traits Korean Cattle
2016/4/25
Leptin is the hormone product of the obese gene and is synthesized and secreted predominantly by white adipocytes and relates to the feedback system that regulates long-term body fat weight and compos...
Analysis on Association of a SNP in the Chicken OBR Gene with Growth and Body Composition Traits
Chicken OBR Gene Single Nucleotide Polymorphism (SNP) Abdominal Fat
2016/4/22
Leptin receptor (OBR) is a member of the class I cytokine receptor family. It signals mainly via the JAK/STAT pathway and plays an important role in regulating body energy storage and metabolism. This...
A Y-linked SNP in SRY Gene Differentiates Chinese Indigenous Swamp Buffalo and Introduced River Buffalo
Swamp buffalo River buffalo SRY SNP Genetic diversity Conservation
2016/4/21
The complete coding region sequence of the SRY gene in Chinese swamp buffalo was determined by PCR product sequencing. Comparison of swamp and river buffalo SRY gene sequences revealed a single nucleo...
Association of SNP Marker in IGF-I and MYF5 Candidate Genes with Growth Traits in Korean Cattle
IGF-I MYF5 Candidate Gene Growth Traits Korean Cattle
2016/4/19
Growth rate is one of the economically important quantitative traits that affect carcass quantity in beef cattle. Two genes, bovine insulin-like growth factor I (IGF-I) and myogenic factor 5 (MYF5), w...